This study examines how B2B companies work with sustainable transportation in practice and how these practices affect their marketing strategies. The study focuses both on the company's sustainability work itself and on its relation to marketing. This arises from the growing demand for sustainable transport solutions from customers, legislators, and stakeholders. The research question investigated in this study is: “How do B2B companies work with sustainable transportation in practice, and what significance does this have for their marketing strategies?” The study applies the theories of Resource Based View (RBV), Institutional Theory, Corporate Social Responsibility (CSR), and the Triple Bottom Line (TBL) to answer this research question and to analyze the survey results. A quantitative method was used through a survey. The survey was distributed to 150 companies based on the criteria of being B2B and actively using sustainable transportation. The selection of companies was made through the researchers own networks as well as through internet searches where emails were sent to the companies. The conclusion of the study is that sustainable transportation has an important significance for B2B companies sustainability work and marketing. The study shows that from a marketing perspective, sustainable transportation plays a central role for B2B companies, but its significance is influenced by internal resources and external pressures. Even if not all B2B companies view sustainability as a central issue in their marketing strategies, the work with sustainable transportation is still important due to external requirements and expectations.