In Indigenous and rural communities of the Global South, relationships between humans, water, and life are understood and organized in various ways, with water often viewed as intrinsically linked to land. These resources not only serve the tangible purpose of supporting livelihoods but also form a fundamental basis for intangible aspects such as culture, identity, and epistemic-ontological foundations. In this article, the interconnected rights to both water and land for these communities are conceptualized as "hydro-territorial rights" (HTRs). This concept encompasses the formal and/or customary norms and practices related to the ownership, access, control, and use of both land and water, which are regarded as interrelated entities. Theoretically, this article draws on rights-based critical institutionalism and political ecology approaches to natural resource governance, including the legal-pluralist distinction between de jure rights on paper and de facto rights in practice. The aim is to identify and comparatively analyze contentious situations and conflicts surrounding water and land rights in rural Indigenous contexts across three postcolonial settings in the Global South. Methodologically, we employ a comparative strategy based on theory and literature reviews to examine conflictual hydro-territorial rights situations within selected Indigenous localities in Bolivia, India, and Tanzania. This analysis is complemented by interviews with local actors and observations in these three settings. Among our findings, we highlight both conflicts and temporary alliances between local and external interests, as well as practices and mechanisms related to the colonial legacy. We also explore how contemporary capitalist developmental interventions in these areas have impacted communities' access to and rights over local water and land resources, resulting in significant consequences for local livelihoods and ethno-cultural-territorial identities.

Blooms of filamentous cyanobacteria regularly occur in the Baltic Sea during warm summer months. These blooms can be toxic and interfere with recreational activities. However, the underlying drivers of these events and how their distribution might change in response to future climate conditions remain poorly understood. To investigate this, we applied a multi-realm modeling approach that integrates environmental data from both marine and terrestrial systems, combined with climate projections, to predict future filamentous and nitrogen-fixing cyanobacterial distribution along Sweden's Baltic Sea coast. Our models identified several key factors significantly influencing bloom distribution: terrestrial temperature, precipitation during the wettest quarter, sea surface temperature, nitrate levels, and interactions between landbased and marine environmental variables. Our projections suggest an expansion of filamentous cyanobacteria in the northern Baltic Proper, Bothnian Sea, Bothnian Bay, and Arkona Basin driven by rising land temperatures by 2070, increasing sea surface temperatures by 2100, and declining salinity in specific basins. Overall, the results demonstrate that incorporating environmental data from both land and sea improves predictions of cyanobacterial distribution in coastal Baltic Sea regions. This multi-realm modeling strategy may also prove valuable for forecasting and managing harmful cyanobacterial blooms in other coastal areas experiencing similar environmental challenges.

Denitrifying microbial communities provide an important ecosystem function in aquatic systems. Yet, knowledge on predictive and modeling of these complex and changing communities is limited. The emergently challenging question of how the geographical distribution of denitrifiers responds to ongoing and future environmental change is not yet fully understood. In our study we use metadata-based correlative niche modeling to analyze the geographical distribution of selected putative denitrifiers in the genus Sphingomonas, Mycoplana, Shewanella, and Alteromonas at different predicted environmental conditions and future climatic scenarios across the Baltic Sea. Using the predictive power of an ensemble modeling approach and eight different machine-learning algorithms, habitat suitability and the distribution of the selected denitrifiers were evaluated using geophysical and bioclimatic variables, benthic conditions, and four Representative Concentration Pathway (RCP) trajectories of future global warming scenarios. All algorithms provided successful prediction capabilities both for variable importance, and for habitat suitability with Area Under the Curve (AUC) values between 0.89 and 1.00. Model findings revealed that salinity and nitrate concentrations significantly explained the variation in distribution of the selected denitrifiers. Rising temperatures of 0.8 to 1.8 °C at future RCP60–2050 trajectories are predicted to diminish or eliminate the bioclimatic suitable habitats for denitrifier distributions across the Baltic Sea. Multi-collated terrestrial and marine environmental variables contributed to the successful prediction of denitrifier distributions within the study area. The correlative niche modeling approach with high AUC values presented in the study allowed for accurate projections of the future distributions of the selected denitrifiers. The modeling approach can be used to improve our understanding of how ongoing and predicted future environmental changes may affect habitat suitability for organisms with denitrification capacity across the Baltic Sea.

Denitrification in sediments is a key microbial process that removes excess fixed nitrogen, while dissimilatory nitrate reduction to ammonium (DNRA) converts nitrate to ammonium. Although microorganisms are responsible for essential nitrogen (N) cycling, it is not yet fully understood how these microbially mediated processes respond to toxic hydrophobic organic compounds (HOCs) and metals. In this study, we sampled long-term polluted sediment from the outer harbor of Oskarshamn (Baltic Sea), measured denitrification and DNRA rates, and analyzed taxonomic structure and N-cycling genes of microbial communities using metagenomics. Results showed that denitrification and DNRA rates were within the range of a national reference site and other unpolluted sites in the Baltic Sea, indicating that long-term pollution did not significantly affect these processes. Furthermore, our results indicate an adaptation to metal pollution by the N-cycling microbial community. These findings suggest that denitrification and DNRA rates are affected more by eutrophication and organic enrichment than by historic pollution of metals and organic contaminants.

The reconstruction of bacterial and archaeal genomes from shotgun metagenomes has enabled insights into the ecology and evolution of environmental and host-associated microbiomes. Here we applied this approach to >10,000 metagenomes collected from diverse habitats covering all of Earth’s continents and oceans, including metagenomes from human and animal hosts, engineered environments, and natural and agricultural soils, to capture extant microbial, metabolic and functional potential. This comprehensive catalog includes 52,515 metagenome-assembled genomes representing 12,556 novel candidate species-level operational taxonomic units spanning 135 phyla. The catalog expands the known phylogenetic diversity of bacteria and archaea by 44% and is broadly available for streamlined comparative analyses, interactive exploration, metabolic modeling and bulk download. We demonstrate the utility of this collection for understanding secondary-metabolite biosynthetic potential and for resolving thousands of new host linkages to uncultivated viruses. This resource underscores the value of genome-centric approaches for revealing genomic properties of uncultivated microorganisms that affect ecosystem processes.

In coastal aphotic sediments, organic matter (OM) input from phytoplankton is the primary food resource for benthic organisms. Current observations from temperate ecosystems like the Baltic Sea report a decline in spring bloom diatoms, while summer cyanobacteria blooms are becoming more frequent and intense. These climate-driven changes in phytoplankton communities may in turn have important consequences for benthic biodiversity and ecosystem functions, but such questions are not yet sufficiently explored experimentally. Here, in a 4-week experiment, we investigated the response of microeukaryotic and bacterial communities to different types of OM inputs comprising five ratios of two common phytoplankton species in the Baltic Sea, the diatom Skeletonema marinoi and filamentous cyanobacterium Nodularia spumigena. Metabarcoding analyses on 16S and 18S ribosomal RNA (rRNA) at the experiment termination revealed subtle but significant changes in diversity and community composition of microeukaryotes in response to settling OM quality. Sediment bacteria were less affected, although we observed a clear effect on denitrification gene expression (nirS and nosZ), which was positively correlated with increasing proportions of cyanobacteria. Altogether, these results suggest that future changes in OM input to the seafloor may have important effects on both the composition and function of microbenthic communities.

Benthic ecosystems have come under intense pressure, due to eutrophication-driven oxygen decline and industrial metal contamination. One of the most toxic metals is Cadmium (Cd), which is lethal to many aquatic organisms already at low concentrations. Denitrification by facultative anaerobic microorganisms is an essential process to transform, but also to remove, excess nitrate in eutrophied systems. Cd has been shown to decrease denitrification and sequester free sulfide, which is available when oxygen is scarce and generally inhibits complete denitrification (i.e. N2O to N2). In polluted sediments, an interaction between oxygen and Cd may influence denitrification and this relationship has not been studied. For example, in the Baltic Sea some sediments are double exposed to both Cd and hypoxia. In this study, we examined how the double exposure of Cd and fluctuations in oxygen affects denitrification in Baltic Sea sediment. Results show that oxygen largely regulated N2O and N2 production after 21 days of exposure to Cd (ranging from 0 to 500 μg/L, 5 different treatments, measured by the isotope pairing technique (IPT)). In the high Cd treatment (500 μg/L) the variation in N2 production increased compared to the other treatments. Increases in N2 production are suggested to be an effect of 1) enhanced nitrification that increases NO3 − availability thus stimulating denitrification, and 2) Cd successfully sequestrating sulfide (yielding CdS), which allows for full denitrification to N2. The in situ field sediment contained initially high Cd concentrations in the pore water (∼10 μg/L) and microbial communities might already have been adapted to metal stress, making the effect of low Cd levels negligible. Here we show that high levels of cadmium pollution might increase N2 production and influence nitrogen cycling in marine sediments. © 2019 The Authors

The Baltic Sea is heavily affected by eutrophication caused by nutrient overload, climate, and infrequency in major inflow events, resulting in widespread areas of oxygen depleted waters and sediments. A Major Baltic Inflow event (MBI) brings saline and oxygenated Atlantic Ocean water into the Baltic Sea, as occurred in 2014. Using a theoretical framework based on metapopulation and metacommunity theory we predicted a transition of the sediment bacterial community after the MBI, from a more heterogeneous community pattern driven by local colonisation-extinction dynamics towards a more pronounced environmental gradient but with reduced beta-diversities. Community diversity patterns before and after the MBI were investigated by 16S rRNA gene sequencing of samples from 42 Baltic Sea environmental monitoring stations. Results showed strong metapopulation dynamics with many satellite and few core taxa. NMDS-ordination showed distinct geographical clustering. After the MBI, alpha-diversity increased, beta-diversity decreased and a significant distance-decay relationship developed. Changes in community composition correlated significantly with changes in oxygen and salinity from 2010 to 2015. Our results indicate strong metapopulation and metacommunity structuring of sediment bacterial diversity and composition in the Baltic Sea and how movements of large-scale water bodies affect bacterial communities through changes in large-scale environmental gradients and dispersal patterns.

In this work, we analyzed the community structure and metabolic potential of sediment microbial communities in high-latitude coastal environments subjected to low to moderate levels of chronic pollution. Subtidal sediments from four low-energy inlets located in polar and subpolar regions from both Hemispheres were analyzed using large-scale 16S rRNA gene and metagenomic sequencing. Communities showed high diversity (Shannon's index 6.8 to 10.2), with distinct phylogenetic structures (<40% shared taxa at the Phylum level among regions) but similar metabolic potential in terms of sequences assigned to KOs. Environmental factors (mainly salinity, temperature, and in less extent organic pollution) were drivers of both phylogenetic and functional traits. Bacterial taxa correlating with hydrocarbon pollution included families of anaerobic or facultative anaerobic lifestyle, such as Desulfuromonadaceae, Geobacteraceae, and Rhodocyclaceae. In accordance, biomarker genes for anaerobic hydrocarbon degradation (bamA, ebdA, bcrA, and bssA) were prevalent, only outnumbered by alkB, and their sequences were taxonomically binned to the same bacterial groups. BssA-assigned metagenomic sequences showed an extremely wide diversity distributed all along the phylogeny known for this gene, including bssA sensu stricto, nmsA, assA, and other clusters from poorly or not yet described variants. This work increases our understanding of microbial community patterns in cold coastal sediments, and highlights the relevance of anaerobic hydrocarbon degradation processes in subtidal environments.