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Elabbas, Lubna
Publications (2 of 2) Show all publications
Porseryd, T., Reyhanian Caspillo, N., Volkova, K., Elabbas, L., Källman, T., Dinnétz, P., . . . Porsch Hällström, I. (2018). Testis transcriptome alterations in zebrafish (Danio rerio) with reduced fertility due to developmental exposure to 17α-ethinyl estradiol. General and Comparative Endocrinology, 262, 44-58
Open this publication in new window or tab >>Testis transcriptome alterations in zebrafish (Danio rerio) with reduced fertility due to developmental exposure to 17α-ethinyl estradiol
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2018 (English)In: General and Comparative Endocrinology, ISSN 0016-6480, E-ISSN 1095-6840, Vol. 262, p. 44-58Article in journal (Refereed) Published
Abstract [en]

17α-Ethinylestradiol (EE2) is a ubiquitous aquatic contaminant shown to decrease fish fertility at low concentrations, especially in fish exposed during development. The mechanisms of the decreased fertility are not fully understood. In this study, we perform transcriptome analysis by RNA sequencing of testes from zebrafish with previously reported lowered fertility due to exposure to low concentrations of EE2during development. Fish were exposed to 1.2 and 1.6 ng/L (measured concentration; nominal concentrations 3 and 10 ng/L) of EE2 from fertilization to 80 days of age, followed by 82 days of remediation in clean water. RNA sequencing analysis revealed 249 and 16 genes to be differentially expressed after exposure to 1.2 and 1.6 ng/L, respectively; a larger inter-sample variation was noted in the latter. Expression of 11 genes were altered by both exposures and in the same direction. The coding sequences most affected could be categorized to the putative functions cell signalling, proteolysis, protein metabolic transport and lipid metabolic process. Several homeobox transcription factors involved in development and differentiation showed increased expression in response to EE2 and differential expression of genes related to cell death, differentiation and proliferation was observed. In addition, several genes related to steroid synthesis, testis development and function were differentially expressed. A number of genes associated with spermatogenesis in zebrafish and/or mouse were also found to be differentially expressed. Further, differences in non-coding sequences were observed, among them several differentially expressed miRNA that might contribute to testis gene regulation at post-transcriptional level. This study has generated insights of changes in gene expression that accompany fertility alterations in zebrafish males that persist after developmental exposure to environmental relevant concentrations of EE2 that persist followed by clean water to adulthood. Hopefully, this will generate hypotheses to test in search for mechanistic explanations.

Place, publisher, year, edition, pages
Academic Press, 2018
National Category
Other Biological Topics Environmental Sciences
Research subject
Baltic and East European studies
Identifiers
urn:nbn:se:sh:diva-29441 (URN)10.1016/j.ygcen.2018.03.011 (DOI)000430995100006 ()29526718 (PubMedID)2-s2.0-85044314644 (Scopus ID)1556/42/2011 (Local ID)1556/42/2011 (Archive number)1556/42/2011 (OAI)
Funder
The Foundation for Baltic and East European Studies, A065-2011Stockholm County Council, 806/3.1.1/2014
Note

Som manuskript i avhandling. As manuscript in dissertation.

Available from: 2016-02-02 Created: 2016-02-04 Last updated: 2020-07-17Bibliographically approved
Porseryd, T., Kellner, M., Reyhanian, N., Volkova, K., Elabbas, L., Ullah, S., . . . Porsch Hällström, I. (2017). Combinatory effects of low concentrations of 17α-etinylestradiol and citalopram on non-reproductive behavior in adult zebrafish (Danio rerio). Aquatic Toxicology, 193, 9-17
Open this publication in new window or tab >>Combinatory effects of low concentrations of 17α-etinylestradiol and citalopram on non-reproductive behavior in adult zebrafish (Danio rerio)
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2017 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 193, p. 9-17Article in journal (Refereed) Published
Abstract [en]

Sewage treatment plant effluents contain a complex mixture of pharmaceuticals, personal care products and industrial chemicals, thus exposing aquatic organisms. Still, the consequences of exposure to combinations of different classes of drugs is largely unknown. In this study, we expose adult zebrafish (Danio rerio) males and females to low, environmentally relevant concentrations of the endocrine disrupting chemical 17α-ethinyl estradiol (EE2) and the selective serotonin re-uptake inhibitor (SSRI) citalopram, alone and in combination, and analyse three non-reproductive behaviours of importance for population fitness.

Two weeks exposure to 0.1 and 0.5 ng/LEE2 resulted in increased anxiety in males in the scototaxis (light/dark preference) test. Significantly longer latency periods before entering the white zone and fewer visits in the white zone were observed in males exposed to both 0.1 and 0.5 ng/LEE2 compared to unexposed males. No significant effects of citalopram alone (0.1 and 0.5 µg/L) were observed in the scototaxis test. The combined exposures (0.1 ng/L EE2 + 0.1 µg/L citalopram and 0.5 ng/L EE2 + 0.5 µg/L citalopram) resulted in abolishment of the anxiogenic effects of EE2, with significantly shorter latency period (low dose) and more transitions to white (high and low dose) than in fish exposed to EE2 alone. No significant effects of either EE2, citalopramor the combination of the two were observed in females. In the novel tank test, significantly more transitions to the upper half of the tank were observed in males exposed to 0.1 µg/L citalopram alone compared to unexposed males while males exposed to 0.1 ng/lEE2 had significantly shorter latency period to enter the upper half. Exposure to the combination of the two low concentrations did, however, result in a significantly longer latency and fewer transitions to upper half compared to both control, EE2- and citalopram-exposed males. These males also spent significantly less time in the upper half than the fish exposed to 0.1 ng/l EE2 or 0.1 µg/l citalopram alone. No significant effects on novel tank behaviour were observed in females or males exposed to the higher concentrations. In the shoaling test, males exposed to 0.1 µg/L citalopram and females exposed to 0.5 ng/l EE2 made significantly fewer transitions away from peers while males exposed to 0.1 µg/L citalopram + 0.1 ng/l EE2 performed significantly more transitions than the fish exposed to 0.1 µg/L citalopram alone.

In conclusion, this study shows that very low concentrations ofEE2, at or slightly above the predicted noeffect concentration (NOEC), affects anxiety in zebrafish males. Furthermore, citalopram, in spite of marginal effect of its own at such low levels, counteracts the response to EE2. This study represents an initial effort to understand the effects on water-living organisms of the cocktails of anthropogenic substances contaminating aquatic environments.

Place, publisher, year, edition, pages
Elsevier, 2017
National Category
Environmental Sciences
Research subject
Environmental Studies
Identifiers
urn:nbn:se:sh:diva-32427 (URN)10.1016/j.aquatox.2017.10.001 (DOI)000417658800002 ()29017090 (PubMedID)2-s2.0-85030678324 (Scopus ID)
Funder
Stockholm County Council, 806/3.1.1/2014The Foundation for Baltic and East European Studies, 1352/3.1.1/2015
Note

As manuscript in dissertation with title: Combinatory effects of low-dose 17alpha-etinyl estradiol and citalopram on behavior in adult zebrafish (Danio rerio)

Available from: 2017-04-19 Created: 2017-04-19 Last updated: 2020-03-24Bibliographically approved
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