Microbial communities comprise immense diversity, mediating numerous ecosystem functions such as nitrogen transformation in aquatic ecosystems. Yet, how microbial communities and ecosystem functions respond to changes in environmental conditions at different spatial scales in aquatic habitats is not fully understood. This thesis investigates the effect of changes in environmental conditions at different spatial scales on Baltic Sea microbial diversity and nitrogen transformation, specifically denitrification, with a focus on biodiversity-ecosystem function relationship. Two spatial scale settings were examined. A small spatial scale setting was examined with experimental mesocosms of benthic sediment of different salinity and dissolved oxygen levels and at different spatial arrangements, and an incubation experiment conducted with benthic sediments from two different aquatic habitats enriched with 15N nitrate isotope. Microbial diversity, denitrification gene transcript abundances, and potential denitrification rate were analyzed. At a large spatial scale of the Baltic Sea benthic and coastal seascape, a metadata-based modeling approach was employed to investigate how current environmental conditions and future climatic change scenarios affect the predicted spatial distribution of key taxa of denitrifiers and nitrogen-fixing filamentous cyanobacteria. Results showed that salinity and dissolved oxygen levels significantly influenced denitrification capacity, according to analyses of transcript abundances of nirS and nosZ genes, which was mainly driven by habitat environmental conditions rather than habitat spatial arrangement. Bacterial community composition in experimental mesocosms was significantly affected by salinity. Findings from the small scale mesocosms experiments indicate that salinity can be an important driver of microbial denitrification capacity and affect microbial diversity in studied mesocosms. At large coastal and seascape scales, multi-realm environmental variables from both marine and terrestrial domains had significant impacts on the predicted distribution of the studied microbial taxa. The predicted distribution under different global warming trajectories indicated that rising temperatures in year2050 and 2100 risk reducing the current bio-climatically suitable areas of the studied denitrifiers and filamentous cyanobacteria across the Baltic Sea. The studies with experimental mesocosms and modeling approaches presented in this thesis contribute knowledge and novel predictions on the spatial response of ecosystem function and microbial communities to ongoing and future environmental changes in the Baltic Sea.

Denitrifying microbial communities provide an important ecosystem function in aquatic systems. Yet, knowledge on predictive and modeling of these complex and changing communities is limited. The emergently challenging question of how the geographical distribution of denitrifiers responds to ongoing and future environmental change is not yet fully understood. In our study we use metadata-based correlative niche modeling to analyze the geographical distribution of selected putative denitrifiers in the genus Sphingomonas, Mycoplana, Shewanella, and Alteromonas at different predicted environmental conditions and future climatic scenarios across the Baltic Sea. Using the predictive power of an ensemble modeling approach and eight different machine-learning algorithms, habitat suitability and the distribution of the selected denitrifiers were evaluated using geophysical and bioclimatic variables, benthic conditions, and four Representative Concentration Pathway (RCP) trajectories of future global warming scenarios. All algorithms provided successful prediction capabilities both for variable importance, and for habitat suitability with Area Under the Curve (AUC) values between 0.89 and 1.00. Model findings revealed that salinity and nitrate concentrations significantly explained the variation in distribution of the selected denitrifiers. Rising temperatures of 0.8 to 1.8 °C at future RCP60–2050 trajectories are predicted to diminish or eliminate the bioclimatic suitable habitats for denitrifier distributions across the Baltic Sea. Multi-collated terrestrial and marine environmental variables contributed to the successful prediction of denitrifier distributions within the study area. The correlative niche modeling approach with high AUC values presented in the study allowed for accurate projections of the future distributions of the selected denitrifiers. The modeling approach can be used to improve our understanding of how ongoing and predicted future environmental changes may affect habitat suitability for organisms with denitrification capacity across the Baltic Sea.

To combat the rising global temperature Sweden have signed the Paris Agreement to limit the release of CO2 and keep the temperature to a minimum increase of 1,5 degrees. A transformation of today’s energy system towards renewable energy is paramount to this endeavour, which in part relies on public acceptance where media plays an important role inshaping public acceptance. This study aims to map acceptance of renewable energy sources and draws on framing theory, which states that the media focuses attention on certain events and gives information meaning, in a sense how something is presented to the audience influences their choices in how to process said information. As this is a qualitative study, 49 articles about bio-, geothermal, solar, water and wind energy from Sweden’s four major newspapers, Aftonbladet, Dagens Nyheter, Expressen and Svenska Dagbladet, have been gathered from the Svenska dagstidningar database and examined through frame analysis. Media framing contributing to public acceptance was bio- and solar energy while water subtracted from it. Wind energy were both contributing and subtracting from acceptance while geothermal lacked articles entirely, prohibiting it from entering public discourse altogether. Meanwhile the biggest deciding factor towards framing acceptance for renewable energy depended largely on the newspaper.

In coastal aphotic sediments, organic matter (OM) input from phytoplankton is the primary food resource for benthic organisms. Current observations from temperate ecosystems like the Baltic Sea report a decline in spring bloom diatoms, while summer cyanobacteria blooms are becoming more frequent and intense. These climate-driven changes in phytoplankton communities may in turn have important consequences for benthic biodiversity and ecosystem functions, but such questions are not yet sufficiently explored experimentally. Here, in a 4-week experiment, we investigated the response of microeukaryotic and bacterial communities to different types of OM inputs comprising five ratios of two common phytoplankton species in the Baltic Sea, the diatom Skeletonema marinoi and filamentous cyanobacterium Nodularia spumigena. Metabarcoding analyses on 16S and 18S ribosomal RNA (rRNA) at the experiment termination revealed subtle but significant changes in diversity and community composition of microeukaryotes in response to settling OM quality. Sediment bacteria were less affected, although we observed a clear effect on denitrification gene expression (nirS and nosZ), which was positively correlated with increasing proportions of cyanobacteria. Altogether, these results suggest that future changes in OM input to the seafloor may have important effects on both the composition and function of microbenthic communities.

The Earth’s permanently cold biosphere is known to harbour abundant microbial biomass and represents a rich resource for the discovery of novel cold-adapted microorganisms, many of which form part of the ‘microbial dark matter’ which cannot be analysed using traditional culture-dependent approaches. The recent development of metagenomics and related multi-omics strategies has provided a means by which entire microbial communities can be studied directly, without the prerequisite of culturing. The advancement of the ‘omic’ methods is directly linked to recent progress in high-throughput sequencing, robust data processing capabilities and the application of cutting-edge analytical tools for high-throughput detection of biomolecules. The combined application of these tools and strategies has provided an unprecedented access to the structure and potential function of microbial communities in cold environments, providing increasingly comprehensive insights into the taxonomic richness and functional capacity of the indigenous microorganisms. Applications of ‘omic’ strategies have enhanced our understanding of psychrophilic adaptation mechanisms, revealing the versatility and adaptability of life in the ‘cryosphere’. In addition to the predicted roles of psychrophiles in biogeochemical cycling, recent multi-omic studies have further emphasised the importance of the ‘cryosphere’ in influencing global atmospheric conditions. Finally, metagenomic bioprospecting of cold environments has yielded a variety of novel bioactive molecules including novel ‘psychrozymes’, with a wide range of potential industrial and biotechnological applications. Here, we have provided an overview of recent developments in metagenomic technologies and their application in the study of the cold biosphere.

Many factors affect the habitat selection for animal species, which in turn may greatly affect their distribution in different ecosystems. Understanding the processes that affect habitat selection is also critical for guiding and managing conservation initiatives. Our study aimed to assess the habitat selection by free-ranging Spiny-tailed lizard (Uromastyx aegyptia) by analyzing a geospatial data connecting its burrow parameters to different habitat characteristics within selected sites in Hail region, Saudi Arabia. We examined evidence and patterns of significant spatial clustering for (366) active burrows by linking their parameters (burrow entrance size, burrow entrance width and burrow entrance height), their reference geographical locations and, two habitat characteristics defined by soil type and vegetation cover. The objective of the analysis was to increase the understanding on the burrows aggregation process in the space and, to describe its possible relation to other spatial habitat configurations. Analysis of distances based on the Nearest Neighbor Index (NNI) and hotspots detection in Nearest neighbor hierarchical clustering (Nnh) suggested twelve (12) spatial clusters located within the study area. In addition, a spatial ordinary least square (OLS) and Poisson regression models revealed significant effects of soil type and vegetation cover on burrow parameters (OLS, p < 0.05; Poisson, p < 0.001), which indicate a strong association between burrows parameters and habitats characteristics. Findings from the study also suggest that other factors such as elevations, highways, and human settlement concentration spots could possibly play a major role in defining burrow spatial aggregation and furthermore have a significant impact on habitat selection.

The value and importance of lectures in higher education is part of a modern education discourse worldwide. This study aims to estimate the importance of lectures for prospective teachers of kindergarten, preschool and early primary school. We analysed academic achievements of prospective teachers who had either mandatorily or voluntarily attended lectures in the subject of teaching and learning mathematics. Students’ examination grades in a maths course with mandatory or voluntary lecture attendance were analysed with a logistic model testing the association between lecture attendance requirement and grades. We show that mandatory lecture attendance (1) more than double the odds of students receiving a pass grade when their situated and tacit knowledge was examined and (2) quadrupled the odds of students achieving the highest grade (pass with distinction) when both their understanding of elementary mathematics and their situated and tacit knowledge of teaching and learning mathematics were examined. Our study provides evidence for a significant positive role of lecture attendance for students acquiring skills in Teaching and Learning Mathematics. While attending lectures students receive situated tacit knowledge of the subject which is otherwise difficult, if not impossible, for them to obtain in a different way. The observed improvement may have an additional positive effect in being a step towards overcoming a maths anxiety, which is otherwise relatively common among prospective teachers.

Digitalization and multidisciplinary approach are the new trends in Teaching and Learning theory and practice. The article lays emphasis on mathematics teaching and learning knowledge, based on Action Research Theory, New Learning Theory and Variation Theory. Preservice early elementary school teachers created audiovisual video films (AV) and Podcasts (PD) lessons integrating Mathematics, Music and Language during a university course in teacher education. These multidisciplinary lessons were used as intelligent tutors in a flipped classroom model. The lessons are dealing with proportionality, one of the most difficult items in elementary school mathematical curriculum. For explaining proportionality, the preservice teachers have used multiple representations such as manipulatives, fiction stories, videogame Minecraft, “Smash up” in tennis table, musical intervals, self-composed, self-written and self-performed songs about mathematical rules in easy to remember rap-style. Preservice teachers have shown their AV or PD lessons to elementary school students followed by joint discussions and exercisers in solving of mathematical problems. Preservice teachers analyzed their work in an essay-form report describing creation of their intelligent tutors for elementary school students’ knowledge development in mathematics, music and language, including pronunciation, explanation and understanding of mathematical terminology. The multipart flipped classroom approach promoted prospective teachers’ professional growth. For future analysis the described multipart flipped classroom model can be further developed in international collaboration.

Nutrient loads from inland sources to the Baltic Sea and adjacent inland waters need to be reduced in order to prevent eutrophication and meet requirements of the European Water Framework Directive (WFD) and the Baltic Sea Action Plan (BSAP). We here investigate the spatial implications of using different possible criteria for reducing water-borne phosphorous (P) loads in the Northern Baltic Sea River Basin District (NBS-RBD) in Sweden. Results show that most catchments that have a high degree of internal eutrophication do not express high export of P from their outlets. Furthermore, due to lake retention, lake catchments with high P-loads per agricultural area (which is potentially of concern for the WFD) did not considerably contribute to the P-loading of the Baltic Sea. Spatially uniform water quality goals may, therefore, not be effective in NBS-RBD, emphasizing more generally the need for regional adaptation of WFD and BSAP-related goals.

This paper makes a contribution to the debate that has been described as a tension between instrumental and emancipatory educational objectives in environment and sustainability education. The contribution involves a methodological approach (introd-) using the concept ‘dislocatory moments’, to identify and analyse moments in classroom practice that address educational objectives relating to ‘change for sustainability’ and ‘thinking and acting independently’. A case of business education, when ‘sustainable development’ is integrated in a series of lessons, is used to exemplify the approach involving analysis of the emergence and closure of a dislocatory moment and the change of logics that occur. The illustrative case shows how room for subjectivity and change can be intertwined in educational practice. It is suggested that the methodological approach could be used in empirical research of classroom practice to further knowledge about the kind of situations that contribute to ‘business as un-usual’ without compromising emancipatory education ideals.

Calls for the inclusion of sustainable development in the business curriculum have increased significantly in the wake of the financial crisis and increased concerns around climate change. This has led to the appearance of new initiatives and the development of new teaching approaches. This thesis explores business education at the upper secondary school level in Sweden following the inclusion of the concept of sustainable development in the curriculum. Drawing on poststructuralist discourse theory, the overarching purpose is to identify the roles of a responsible business person that are articulated in business education and to discuss how these roles could enable students to address sustainability issues. The thesis consists of four studies, based on textbook analyses, teacher interviews and classroom observations. Three categories of roles have been identified, implying that a business person is expected to either adapt to, add or create ethical values. These three categories are compared with the roles indicated in the environmental discourses constructed by Dryzek and the responsibility regimes developed by Pellizzoni. Drawing on Dryzek’s and Pellizzoni’s reasoning about which qualities are important for addressing sustainability issues, it is concluded that the roles identified in the studies could mean that students are unequipped (the adapting role), ill-equipped (the adding role) or better equipped (the creating role) to address uncertain and complex sustainability issues. The articles include empirical examples that illustrate how and in which situations specific roles are articulated, privileged or taken up. The examples also indicate how the scope for business students’ subjectivities are facilitated or hampered. It is suggested that the illustrative empirical examples could be used for critical reflection in order to enhance students’capabilities of addressing uncertain and complex sustainability issues and to improve educational quality in terms of scope for subjectivity.

This paper explores various kinds of logics of ‘business education for sustainability’and how these ‘logics’ position the subject business person, based on eight teachers’ reasoning of their own practices. The concept of logics developed within a discourse theoretical framework is employed to analyse the teachers’ reasoning. The analysis takes its starting point in different approaches to how a business ought to or could take responsibility for sustainable development. Different approaches to business ethical responsibilities, in combination with assumptions about how educational content is legitimised and presupposed purposes of education, are used to construct logics of business education for sustainability. In the paper, the results of this analysis are presented as: the logic of profit-, social- or radical-oriented business education. Our results also show how the different logics position the subject business person differently, as one who adapts to, adds or creates ethical values. The results are first discussed in terms of how environmental and social challenges could be dealt with in the future and secondly, considering the risk of de-subjectification with regard to profit-oriented business education, the implications this may have for the educational quality itself.

Humanity is today facing a future with climate change and challenges in the form of periodic heatwaves, increased rainfall and reduced biodiversity. The urbanization processes are driven by expanded population growth and it’s becoming more attractive to live in the cities. This causes the dimension of hard surfaces to increase and consequently the proportion of green spaces sinks. In the construction of housing, a large amount of unused space is added in form of dark areas on roofs. These are not only unused areas on expensive land but create additional problems linked to urbanization in the form of decreased albedo. Furthermore, citizens' willingness to pay has been shown to rise with closeness to green surfaces which can provide recreational and health benefits. Private individuals and property owners would like to profile themselves as sustainable, as the term “sustainability” recently has become an identity-created “status marker”. Sustainable housing can therefore also be a selling factor. Green roofs are not a new invention but have had a renaissance over the years as it has proven to provide the availability to green surfaces for the citizens, stormwater management, heat island effect, and provide healthier air quality. The aim of the study was to examine how the district Norra Djurgårdsstaden in Stockholm, Sweden works with sustainable urban development through green roofs. In this study we have used urban sustainability theory as the green roofs can generate a wealth of economic, ecological and social benefits in urban areas. We have also used our own constructed analysis framework based on previous research and urban sustainability theory. By doing a qualitative content analysis, as well as semi-structured interviews, it has appeared that the social dimension of urban sustainable development in Norra Djurgårdsstaden is overshadowed by the two other aspects; the economic and the ecological. Although the sustainability work in Norra Djurgårdsstaden has taken all three aspects; economic, ecological and social into account when it comes to planning of the district, the social aspect is often neglected.

Human development continues to use up more physical space in the natural world, threatening the natural habitats of many organisms. To combat the loss of biodiversity science needs to explore what landscape features are important for different organisms so that we can incorporate these into the modern environment. As bats play an important role in many ecosystems and can reflect changes through trophic levels, analyzing their preferred habitats can help planners improve biological diversity of the urban habitat. Using acoustically identified bat sightings from Artportalen.se for the years 2017-2018, this paper studied the habitats of bats in Sweden. Through multiple regression analysis we examine the response in abundance and/or diversity of bats to physical and socio-cultural attributes of the urban habitat. We examined a total of 10160 bats from 18 species in 418 land cover locales and 306 demographical statistical areas with varying degrees of urbanization. Our results indicate that bat abundance and diversity decrease significantly with higher urbanization while deciduous forests are the most important land cover type for all bats. The results also indicate that wealthier areas have less abundance and diversity even when factoring in population density. Species specific analysis suggested that bat species who are better adapted at foraging in open vegetated landscapes and over water were less susceptible to the negative impacts of the urban habitat. We conclude that diverse habitats with a mixture of open vegetated areas, watercourses and broadleaf forests are the most important land features for a diverse bat fauna along with high connectivity via tree cover and linear landscape elements. If urban planning could incorporate these features into the urban habitat, some of the negative impacts of urbanization could be prevented. 

A sediment record from a small lake in the north-eastern part of the Kamchatka Peninsula has been investigated in a multi-proxy study to gain knowledge of Holocene climatic and environmental change. Pollen, diatoms, chironomids and selected geochemical parameters were analysed and the sediment record was dated with radiocarbon. The study shows Holocene changes in the terrestrial vegetation as well as responses of the lake ecosystem to catchment maturity and multiple stressors, such as climate change and volcanic eruptions. Climate change is the major driving force resulting in the recorded environmental changes in the lake, although recurrent tephra deposition events also contributed. The sediment record has an age at the base of about 10,000 cal yrs BP, and during the first 400 years the climate was cold and the lake exhibited extensive ice-cover during winter and relatively low primary production. Soils in the catchment were poor with shrub alder and birches dominatingthe vegetation surrounding the lake. At about 9600–8900 cal yrs BP the climate was cold and moist, and strong seasonal wind stress resulted in reduced ice-cover and increased primary production. After ca. 8900 cal yrs BP the forest density increased around the lake, runoff decreased in a generally drier climate resulting in decreasedprimary production in the lake until ca. 7000 cal yrs BP. This generally dry climate was interrupted by a brief climatic perturbation, possibly attributed to the 8.2 ka event, indicating increasingly windy conditions with thick snow cover, reduced ice-cover and slightly elevated primary production in the lake. The diatom record shows maximum thermal stratification at ca. 6300–5800 cal yrs BP and indicates together with the geochemical proxies a dry and slightly warmer climate resulting in a high productive lake. The most remarkably change in the catchment vegetation occurred at ca. 4200 cal yrs BP in the form of a conspicuous increase in Siberian dwarf pine (Pinus pumila), indicating a shift to a cooler climate with a thicker and more long-lasting snow cover. Thisvegetational change was accompanied by marked shifts in the diatom and chironomid stratigraphies, which are also indicative of colder climate and more extensive ice-cover.

Reference total nitrogen (TN) concentrations for the Gårdsfjärden estuary in the central Bothnian Sea, which receives discharge from an industrial point-source, have been estimated from diatom assemblages using a transfer function. Sedimentological and diatom evidence imply a good ecological status before 1920 with an assemblage dominated by benthic taxa indicating excellent water transparency, high diatom species richness and less organic sedimentation resulting in homogeneous well oxygenated sediments. A change in the diatom assemblage starts between 1920 and 1935 when the species richness declines and the proportion of planktic taxa increases. Increased organic carbon sedimentation after 1920 led to hypoxic bottom waters, and the preservation of laminae in the sediments. The trend in the reconstructed TN-values agrees with the history of the discharge from the mill, reaching maximum impact during the high discharge between 1945 and 1990. The background condition for TN in Gårdsfjärden is 260-300 μg L^-1, reconstructed until 1920.

A sediment record from the western Gotland Basin, northwestern Baltic Proper, covering the last 1200 years, was investigated for past changes in climate and the environment using diatoms as a proxy. The aim is to compare the environmental conditions reconstructed during Medieval times with settings occurring the last century under influence of environmental stressors like eutrophication and climate change. The study core records more marine conditions in the western Gotland Basin surface waters during the Medieval Climate Anomaly (MCA; 950–1250C.E.), with a salinity of at least 8 psu compared to the present 6.5 psu. The higher salinity together with a strong summer-autumn stratification caused by warmer climate resulted in extensive long-lasting diatom blooms of Pseudosolenia calcar-avis, effectively enhancing the vertical export of organic carbon to the sediment and contributing to benthic hypoxia. Accordingly, our data support that a warm and dry climate induced the extensive hypoxic areas in the open Baltic Sea during the MCA. During the Little ice Age (LIA; 1400–1700C.E.), the study core records oxic bottom water conditions, decreasing salinity and less primary production. This was succeeded during the 20th century, about 1940, by environmental changes caused by human-induced eutrophication. Impact of climate change is visible in the diatom composition data starting about 1975C.E. and becoming more pronounced 2000C.E., visible as an increase of taxa that thrived in stratified waters during autumn blooms typically due to climate warming.

This study aims to investigate and disentangle the impact of land use and climate variability on the Baltic Sea coastal zone from a millennial perspective. To assess the environmental status of the coastal zone we make use of siliceous microfossils (mainly diatoms), stable nitrogen and carbon isotopes, organic carbon accumulation rates, and lithological changes analyzed in a sediment core collected in Gamlebyviken, Swedish east coast, dated to cover the last 3000 years. Changes in land use and vegetation cover are modelled using pollen stratigraphical data to obtain the percentage coverage of coniferous woodland (Pinus and Picea), deciduous woodland, wetland (Cyperaceae), grassland (including Juniperus) and cropland (cereals) while changes in climatic conditions are assessed through well-documented climatic periods that have occurred in the Baltic Sea region. The reconstructed regional vegetation cover shows that already 3000 years ago, humans used the landscape for both animal husbandry (grasslands) and farming (cropland), but the impact on the Baltic coastal waters was minor. The diatom accumulation rates were quite high (similar to 3100-2600 cal yr BP) containing taxa indicative of high nutrient conditions/upwelling, and stable carbon isotopes show that the carbon was produced in the basin but did not result in elevated organic carbon accumulation rates. A gradual change to less marine conditions in Gamlebyviken from about 2500 to 1400 cal yr BP can be attributed to the ongoing land uplift which resulted in a more enclosed embayment with only a narrow inlet area today. The Medieval Climate Anomaly (1000-700 cal yr BP/950-1250 CE) is a time where extensive eutrophication is registered in the open Baltic Sea, but afforestation is recorded between 1000 and 500 cal yr BP and attributed to the expansion of spruce favored by land-use reorganization with a transition from a one-course rotation system to the three-course rotation system fully established in southern Sweden in the 13th century, and only minor environmental change is recorded in the coastal zone. The Little Ice Age is documented in our data between 400 and 250 cal yr BP/1550-1700 CE as a decrease in regional cropland (cereals) cover, possibly indicating years of poor crop harvest, and changes in the Baltic coastal zone are evidenced as low carbon and diatom accumulation rates, increase in benthic diatom taxa (low turbidity), and high abundance in diatom taxa associated with sea ice indicating a cold climate. The most significant changes occurred from about 100 cal yr BP/1850 CE up to present, with a maximum regional cover of grassland and cropland (ca. 35%) at the expense of deciduous woodland, and major changes indicative of a highly eutrophic environment recorded in the coastal zone. Organic carbon accumulation rates peaked in 1968 CE at approximately 134 g C m(2) yr(-1) before subsequently declining to present-day values of 53 g C m(2) yr(-1), mirroring a similar trend observed in diatom accumulation rates. The high organic carbon accumulation rate shows that deep unvegetated accumulation bottoms in the coastal Baltic Sea serve as carbon sinks and are worth exploring for their potential in mitigating climate change. Variation partitioning shows that 26% of the variance in the diatom assemblages is associated with land use changes. The variables grassland, cropland, and stable nitrogen isotopes are accordingly strong predictors of environmental change in the Baltic coastal zone as reflected by the diatom assemblages.

The Integrated Ocean Drilling Program (IODP) expedition 347 cored sediments from different settings of the Baltic Sea covering the last glacial–interglacial cycle. The main aim was to study the geological development of the Baltic Sea in relation to the extreme climate variability of the region with changing ice cover and major shifts in temperature, salinity, and biological communities. Using the Greatship Manisha as a European Consortium for Ocean Research Drilling (ECORD) mission-specific platform, we recovered 1.6 km of core from nine sites of which four were additionally cored for microbiology. The sites covered the gateway to the North Sea and Atlantic Ocean, several sub-basins in the southern Baltic Sea, a deep basin in the central Baltic Sea, and a river estuary in the north.

The waxing and waning of the Scandinavian ice sheet has profoundly affected the Baltic Sea sediments. During theWeichselian, progressing glaciers reshaped the submarine landscape and displaced sedimentary deposits from earlier Quaternary time. As the glaciers retreated they left a complex pattern of till, sand, and lacustrine clay, which in the basins has since been covered by a thick deposit of Holocene, organic-rich clay. Due to the stratified water column of the brackish Baltic Sea and the recurrent and widespread anoxia, the deeper basins harbor laminated sediments that provide a unique opportunity for high-resolution chronological studies.

The Baltic Sea is a eutrophic intra-continental sea that is strongly impacted by terrestrial runoff and nutrient fluxes. The Holocene deposits are recorded today to be up to 50m deep and geochemically affected by diagenetic alterations driven by organic matter degradation. Many of the cored sequences were highly supersaturated with respect to methane, which caused strong degassing upon core recovery. The depth distributions of conservative sea water ions still reflected the transition at the end of the last glaciation from fresh-water clays to Holocene brackish mud. High-resolution sampling and analyses of interstitial water chemistry revealed the intensive mineralization and zonation of the predominant biogeochemical processes. Quantification of microbial cells in the sediments yielded some of the highest cell densities yet recorded by scientific drilling.

Vector-borne diseases are an increasing global threat to humans due to climate changes, elevating the risk of infections transmitted by mosquitos, ticks, and other arthropod vectors. Ixodes ricinus, a common tick in Europe, transmits dangerous tick-borne pathogens to humans. Tick-borne encephalitis (TBE) is a vector-borne disease caused by TBE virus (TBEV). Climate change has contributed to increased tick abundance and incidence of tick-borne diseases, and between 10,000 and 15,000 human TBE cases are reported annually in Europe and Asia. TBEV shows a patchy geographical distribution pattern where each patch represents a natural focus. In nature, TBEV is maintained within the tick-rodent enzootic cycle. Co-feeding is the main route for TBEV transmission from infected to uninfected ticks and for maintenance within the natural foci. The increasing number of TBE cases in Scandinavia highlights the importance of characterizing additional TBEV sequences and of identifying novel natural foci, and in this work we sequenced and phylogenetically characterized four TBEV strains: Saringe-2009 (from a blood-fed nymph), JP-296 (from a questing adult male), JP-554 (from a questing adult male), and Mandal-2009 (from a pool of questing nymphs, n = 10). Mandal-2009 represents a TBEV genome from a natural focus in southern Norway. Saringe-2009 is from a natural endemic focus in northern Stockholm, Sweden, and JP-296 and JP-554 originate from a natural focus “Torö” in southern Stockholm. In addition, we have studied the effect of different biotic and abiotic factors on population dynamics of I. ricinus in southern Stockholm and observed significant spatiotemporal variations in tick activity patterns. Seasonal synchrony of immature stages and total tick abundance are important factors for the probability of horizontal transmission of TBEV among co-feeding ticks. We found that the probability of co-occurrence of larvae, nymphs, and female adults was highest during early summer whereas increasing vegetation height and increasing amounts of forest and open water around the study sites had a significant negative effect on co-occurrence of larvae, nymphs, and female adults.

The proximal part of the 3 ́non-coding region (3 ́NCR) of TBEV contains an internal poly(A) tract, and genomic analysis of Saringe-2009 revealed variability in the poly(A) tract indicating the existence of different variants within the TBEV pool of Saringe-2009. Like other RNA viruses, TBEV exists as swarms of unique variants called quasispecies. Because Saringe-2009 came from an engorged nymph that had been feeding on blood for >60 h, we propose that Saringe-2009 represents a putative shift in the TBEV pool when the virus switches from ectothermic/tick to endothermic/mammalian environments. We investigated the role of poly(A) tract variability in replication and virulence of TBEV by generating two infectious clones of the TBEV strain Toro-2003, one with a short/wild-type (A)3C(A)6 poly(A) tract and one with a long (A)3C(A)38 poly(A) tract. The infectious clone with the long poly(A) tract showed poor replication in cell culture but was more virulent in C57BL/6 mice than the wild-type clone. RNA folding predictions of the TBEV genomes suggested that insertion of a long poly(A) tract abolishes a stem loop structure at the beginning of the 3 ́NCR. Next generation sequencing (NGS) analysis of the TBEV genomes after passaging in cell culture and/or mouse brain revealed molecular determinants and quasispecies structure that might contribute to the observed differences in virulence. Our findings suggest that the long poly(A) tract imparts instability to the TBEV genome resulting in higher quasispecies diversity that in turn contributes to TBEV virulence. Phylogenetic analysis of Saringe-2009, JP-296, JP-554, and Mandal-2009 predicted a strong evolutionary relationship among the four strains. They clustered with Toro-2003, the first TBEV strain from Torö, demonstrating a Scandinavian clade. Except for the proximal part of the 3 ́NCR, TBEV is highly conserved in its genomic structure. Genomic analysis revealed that Mandal-2009 contains a truncated 3 ́NCR similar to the highly virulent strain Hypr, whereas JP-296 and JP-554 have a genomic organization identical to Toro-2003, the prototypic TBEV strain from the same natural focus. NGS revealed significantly higher quasispecies diversity for JP-296 and JP-554 compared to Mandal-2009. In addition, single nucleotide polymerphism (SNP) analysis showed that 40% of the SNPs were common between quasispecies populations of JP-296 and JP-554, indicating the persistence and maintenance of TBEV quasispecies within the natural focus.

Taken together, these findings indicate the importance of environmental factors for the occurrence pattern of the different life-stages of the tick vector, which are important for the persistence of TBEV in nature. Our findings also show that the selection pressure exerted by specific host also affects the population structure of the TBEV quasispecies. In addition, our results further demonstrate that the evolution of quasispecies has effect on TBEV virulence in mice.

Every year, tick-borne encephalitis virus (TBEV) causes severe central nervous system infection in 10 000 to 15 000 people in Europe and Asia. TBEV is maintained in the environment by an enzootic cycle that requires a tick vector and a vertebrate host, and the adaptation of TBEV to vertebrate and invertebrate environments is essential for TBEV persistence in nature. This adaptation is facilitated by the error-prone nature of the virus's RNA-dependent RNA polymerase, which generates genetically distinct virus variants called quasispecies. TBEV shows a focal geographical distribution pattern where each focus represents a TBEV hotspot. Here, we sequenced and characterized two TBEV genomes, JP-296 and JP-554, from questing Ixodes ricinus ticks at a TBEV focus in central Sweden. Phylogenetic analysis showed geographical clustering among the newly sequenced strains and three previously sequenced Scandinavian strains, Toro-2003, Saringe-2009 and Mandal-2009, which originated from the same ancestor. Among these five Scandinavian TBEV strains, only Mandal-2009 showed a large deletion within the 3' non-coding region (NCR), similar to the highly virulent TBEV strain Hypr. Deep sequencing of JP-296, JP-554 and Mandal-2009 revealed significantly high quasispecies diversity for JP-296 and JP-554, with intact 3' NCRs, compared to the low diversity in Mandal-2009, with a truncated 3' NCR. Single-nucleotide polymorphism analysis showed that 40% of the single-nucleotide polymorphisms were common between quasispecies populations of JP-296 and JP-554, indicating a putative mechanism for how TBEV persists and is maintained within its natural foci.

Shallow coastal soft bottoms are important carbon sinks. Submerged vegetation has been shown to sequester carbon, increase sedimentary organic carbon (C-org) and thus suppress greenhouse gas (GHG) emissions. The ongoing regression of seagrass cover in many areas of the world can therefore lead to accelerated emission of GHGs. In Nordic waters, seagrass meadows have a high capacity for carbon storage, with some areas being recognized as blue carbon hotspots. To what extent these carbon stocks lead to emission of methane (CH4) is not yet known. We investigated benthic CH4 emission (i.e., net release from the sediment) in relation to seagrass (i.e. Zostera marina) cover and sedimentary C-org content (%) during the warm summer period (when emissions are likely to be highest). Methane exchange was measured in situ with benthic chambers at nine sites distributed in three regions along a salinity gradient from similar to 6 in the Baltic Sea (Finland) to similar to 20 in Kattegat (Denmark) and similar to 26 in Skagerrak (Sweden). The net release of CH4 from seagrass sediments and adjacent unvegetated areas was generally low compared to other coastal habitats in the region (such as mussel banks and wetlands) and to other seagrass areas worldwide. The lowest net release was found in Finland. We found a positive relationship between CH4 net release and sedimentary C-org content in both seagrass meadows and unvegetated areas, whereas no clear relationship between seagrass cover and CH4 net release was observed. Overall, the data suggest that Nordic Zostera marina meadows release average levels of CH4 ranging from 0.3 to 3.0 mu g CH4 m(-2) h(-1), which is at least 12-78 times lower (CO2 equivalents) than their carbon accumulation rates previously estimated from seagrass meadows in the region, thereby not hampering their role as carbon sinks. Thus, the relatively weak CH4 emissions from Nordic Z. marina meadows will not outweigh their importance as carbon sinks under present environmental conditions.

Context Seagrass meadows act as efficient natural carbon sinks by sequestering atmospheric CO2 and through trapping of allochthonous organic material, thereby preserving organic carbon (C-org) in their sediments. Less understood is the influence of landscape configuration and transformation (land-use change) on carbon sequestration dynamics in coastal seascapes across the land-sea interface. Objectives We explored the influence of landscape configuration and degradation of adjacent mangroves on the dynamics and fate of C-org in seagrass habitats. Methods Through predictive modelling, we assessed sedimentary C-org content, stocks and source composition in multiple seascapes (km-wide buffer zones) dominated by different seagrass communities in northwest Madagascar. The study area encompassed seagrass meadows adjacent to intact and deforested mangroves. Results The sedimentary C-org content was influenced by a combination of landscape metrics and inherent habitat plant- and sediment-properties. We found a strong land-to-sea gradient, likely driven by hydrodynamic forces, generating distinct patterns in sedimentary C-org levels in seagrass seascapes. There was higher C-org content and a mangrove signal in seagrass surface sediments closer to the deforested mangrove area, possibly due to an escalated export of C-org from deforested mangrove soils. Seascapes comprising large continuous seagrass meadows had higher sedimentary C-org levels in comparison to more diverse and patchy seascapes. Conclusion Our results emphasize the benefit to consider the influence of seascape configuration and connectivity to accurately assess C-org content in coastal habitats. Understanding spatial patterns of variability and what is driving the observed patterns is useful for identifying carbon sink hotspots and develop management prioritizations.

As globalization and other pressures intensify the economic, social and biophysical connections between people and places, it seems likely that adaptation responses intended to ameliorate the impacts of climate change might end up shifting risks and vulnerability between people and places. Building on earlier conceptual work in maladaptation and other literature, this article explores the extent to which concerns about vulnerability redistribution have influenced different realms of adaptation practice. The review leads us to conclude that the potential for adaptation to redistribute risk or vulnerability is being given only sparse—and typically superficial—attention by practitioners. Concerns about ‘maladaptation’, and occasionally vulnerability redistribution specifically, are mentioned on the margins but do not significantly influence the way adaptation choices are made or evaluated by policy makers, project planners or international funds. In research, the conceptual work on maladaptation is yet to translate into a significant body of empirical literature on the distributional impacts of real-world adaptation activities, which we argue calls into question our current knowledge base about adaptation. These gaps are troubling, because a process of cascading adaptation endeavors globally seems likely to eventually re-distribute risks or vulnerabilities to communities that are already marginalized and vulnerable. We conclude by discussing the implications that the potential for vulnerability redistribution might have for the governance of adaptation processes, and offer some reflections on how research might contribute to addressing gaps in knowledge and in practice.

Habitat loss through land-use change is the most pressing threat to biodiversity worldwide. European semi-natural grasslands have suffered an ongoing decline since the early twentieth century, but we have limited knowledge of how grassland loss has affected biodiversity across large spatial scales. We quantify land-use change over 50-70 years across a 175,000 km(2) super-region in southern Sweden, identifying a widespread loss of open cover and a homogenisation of landscape structure, although these patterns vary considerably depending on the historical composition of the landscape. Analysing species inventories from 46,796 semi-natural grasslands, our results indicate that habitat loss and degradation have resulted in a decline in grassland specialist plant species. Local factors are the best predictors of specialist richness, but the historical landscape predicts present-day richness better than the contemporary landscape. This supports the widespread existence of time-lagged biodiversity responses, indicating that further species losses could occur in the future.

The Baltic Sea offers a diversity of marine ecosystem services which is providing support to nearby human communities. Their functions are jeopardized by terrestrial and aquatic activities which have heavily polluted the sea, necessitating long-term sustainable management. The aim of this bachelor thesis is to provide a scientific contribution to understanding the challenges and opportunities for reaching a good ecological status in the Baltic Sea, as well as insight into the ongoing management practices of Swedish county administrative boards and municipalities. Practices that involve conserving ecosystem services in two different marine protected areas the High Coast and St. Anna-Missjö, both of which have been designated important by a regional convention; the Helsinki Commission. Therefore, the Swedish Agency for Marine and Water Management has adopted a report on which ecosystem services the Baltic Sea provides, and the status of the services in different parts of the sea. This study is based on qualitative methods to gather data by a thorough reading of documents written by the counties and semi-structured interviews of local officials. The data has summarily been categorised and analysed in line with the national agency’s report, then discussed afterwards. The results show that the management practices were heavily emphasised on dealing with activities that lead to eutrophication, unsustainable fishery as well as regulation of polluting substances; where trade-offs and synergy-effects converge that impacts the management of other marine ecosystem services. Hence, it is necessary for officials to network with locals to have sustainable management of the protected areas and their ecosystem services. The officials pointed to the presence of a lack of time, resources and expertise in managing marine environments. Although the agency’s perspective on marine ecosystem services permeates large parts of county administrative boards and municipalities' work with marine management in the High Coast and St. Anna-Missjö. The status and functions of ecosystem services are integrated in their management practices of the protected areas. However, the concepts themselves are more absent and not mentioned explicitly. 

Pharmaceuticals are emerging as environmentally problematic compounds. As they are often not appropriately removed by sewage treatment plants, pharmaceutical compounds end up in surface water environments worldwide at concentrations in the ng to μg L−1 range. There is a need to further explore single compound and mixture effects using e.g. in vivo test model systems. We have investigated, for the first time, behavioral effects in larval zebrafish (Danio rerio) exposed to a binary mixture of an antidepressant drug (citalopram) and a synthetic opioid (tramadol). Citalopram and tramadol have a similar mode of action (serotonin reuptake inhibition) and are known to produce drug-drug interactional effects resulting in serotonin syndrome (SS) in humans. Zebrafish embryo-larvae were exposed to citalopram, tramadol and 1:1 binary mixture from fertilization until 144 h post fertilization. No effects on heart rate, spontaneous tail coiling, or death/malformations were observed in any treatment at tested concentrations. Behavior (hypoactivity in dark periods) was on the other hand affected, with lowest observed effect concentrations (LOECs) of 373 μg L−1 for citalopram, 320 μg L−1 for tramadol, and 473 μg L−1 for the 1:1 mixture. Behavioral EC50 was calculated to be 471 μg L−1 for citalopram, 411 μg L−1 for tramadol, and 713 μg L−1 for the 1:1 mixture. The results of this study conclude that tramadol and citalopram produce hypoactivity in 144 hpf zebrafish larvae. Further, a 1:1 binary mixture of the two caused the same response, albeit at a higher concentration, possibly due to SS.

Hepatitis C virus (HCV) affects up to 45,000 people in Sweden today. Although it is a very treatable disease, the prevalence of HCV is extremely high within the population of people who inject drugs (PWID). This study examines the direct effect of needle exchange programs (NEPs) on HCV rates in Sweden. Previous research has shown that NEPs reduce the transmission of other blood-borne diseases among PWID. Using an interrupted time series (ITS) analysis, this study investigates if there are statistically significant differences between HCV rates in Swedish counties before and after the implementation of NEPs. The study also investigates via linear regression to see if there is a relationship between sterile injecting equipment (needles and syringes) dispensed and HCV rates in the counties where NEPs exist. While there has been a steady decrease in HCV rates across the country as a whole, the ITS analyses show no statistically significant differences in HCV rates due to the opening of NEPs. Because of the relatively recent introduction of NEPs in Sweden, more data points post-intervention may be needed before we can truly see the effect they have on regional HCV rates. There is also no relationship between the number of needles and syringes dispensed and county HCV rates. However, Sweden falls far short of the 300 syringes/needles per user per year recommendation of the World Health Organization at this time. Standardized data collection and further research can help answer these questions more clearly. 

Per-and polyfluoroalkyl substances (PFAS) is a collective name for approximately 4700 synthetic chemicals ubiquitous in the aquatic environment worldwide. They are used in a wide array of products and are found in living organisms around the world. Some PFAS have been associated with cancer, developmental toxicity, endocrine disruption, and other health effects. Only a fraction of PFAS are currently monitored and regulated and the presence and effects on aquatic organisms of many PFAS are largely unknown. The aim of this study is to investigate the health effects of environmentally relevant concentrations of PFAS on aquatic organisms at different consumer trophic levels through a systematic review and meta-analysis. The main result shows that PFAS in concentrations up to 13.5 μg/L have adverse effects on body size variables for secondary consumers. However, no significant effects on liver or gonad somatic indices and neither on fecundity were found. In addition, the results show that there are large research gaps for PFAS effects on different organisms in aquatic environments at environmentally relevant concentrations. Most studies have been performed on secondary consumers and there is a substantial lack of studies on other consumers in aquatic ecosystems.

The Baltic Sea is a geologically and evolutionarily young part of the coastal ocean that experienced, in its past, several severe environmental changes. In its present state, the Baltic Sea is characterized by both horizontal and vertical gradients of environmental conditions. As a huge estuary, it shows a west to east/south to north surface salinity gradient from 24 in Kattegat to nearly freshwater in the Bothnian Bay. The vertical salinity and oxygen gradients result in stratification which causes hypoxic and sulfidic anoxic conditions in deep basins. These gradient systems are impacted by natural and anthropogenic changes due to physico-chemical driving forces, varying over time and space. Gradient environments produce an imprint on both the structure and function of the biological systems and influence biogeochemical cycling. Besides, coastal seas in general and the Baltic Sea in particular, experience constant and direct influence from land with consequences to matter and energy cycles, biogeochemical interactions, energy fluxes, and sediment dynamics. “Living along gradients: past, present, future” in the Baltic are today’s very important aspects that rise questions like which of the effects we are detecting occur naturally, and which are driven by human activities. Deciphering past environmental changes and their causes provide keys to understand and simulate possible future scenarios, all of which should rise societal awareness and implementation of appropriate marine and coastal policies. Present-day knowledge on the dynamics of gradient systems, on the processes that affect the coastal sea environment, the results of interaction between coastal seas and society, the detection or reconstruction of past and present changes on time scales from inter-annual to millennial, and future change models are summarized here, with the idea to stimulate scientific exchange on most complex questions, addressing them from different perspectives.

The aim of this thesis is to identify potential routes of participation in environmentally sustainable changes of the Swedish meat production and consumption. Changes are needed as meat production and consumption have been linked to serious environmental problems, such as climate change, biodiversity loss, and land use change. Scientists, international organizations, and Swedish government authorities have identified individual consumer responsibility as key in making that change happen. The public is to be informed and educated to make more environmentally sustainable choices as meat consumers, and become more supportive of policy instruments. This strategy, which mirrors the dominant approach to solving environmental problems, is suggested by government authorities despite their suspicion that media should have made most Swedes aware of the environmental impacts of meat.

In this thesis potential participatory routes are identified through the analysis of Swedish news media and audience framings of meat production and consumption. Media framing is studied as an important source of information, and perhaps motivation, crucial in the individualized consumer responsibility approach. The media framing is studied through content analysis of mainstream and alternative radical newspapers. The audiences’ framing of meat may be influenced by media, but also by their everyday experiences, beliefs, values, and opinions. Focus group discussions with reception elements are the methods used for studying how audiences frame meat and use media in the process. The concept of participation is broadened to include passive and active forms to capture in which roles individuals consider to contribute to changing meat production and consumption. It is not self-evident that routes to change must include individual participation, since responsibility may be attributed to other actors, both by media and their audiences.

The results imply only participatory route supported by media and audience framing. It is the one that mirrors the individualized consumer responsibility approach to solving environmental problems. The major barrier to the route is the audiences’ perceived inability to act. In an alternative route supported by both media and audience framing, state centered actors are made responsible for enforcing change. Here, the major barrier is the perceived unlikeliness of powerful actors assuming responsibility. Audiences construct no citizen roles for themselves to participate in. Neither does media, who only address audiences as consumers. Based on these findings it is suggested that the outlook for the individualized responsibility approach to making meat production and consumption environmentally sustainable is gloomy. At least if it the approach is to continuously rely on the information and motivation offered by media.

BACKGROUND: Through functional screening of a fosmid library, generated from a phytopathogen-suppressive soil metagenome, the novel antifungal chitinase-named Chi18H8 and belonging to family 18 glycosyl hydrolases-was previously discovered. The initial extremely low yield of Chi18H8 recombinant production and purification from Escherichia coli cells (21 μg/g cell) limited its characterization, thus preventing further investigation on its biotechnological potential.

RESULTS: We report on how we succeeded in producing hundreds of milligrams of pure and biologically active Chi18H8 by developing and scaling up to a high-yielding, 30 L bioreactor process, based on a novel method of mild solubilization of E. coli inclusion bodies in lactic acid aqueous solution, coupled with a single step purification by hydrophobic interaction chromatography. Chi18H8 was characterized as a Ca(2+)-dependent mesophilic chitobiosidase, active on chitin substrates at acidic pHs and possessing interesting features, such as solvent tolerance, long-term stability in acidic environment and antifungal activity against the phytopathogens Fusarium graminearum and Rhizoctonia solani. Additionally, Chi18H8 was found to operate according to a non-processive endomode of action on a water-soluble chitin-like substrate.

CONCLUSIONS: Expression screening of a metagenomic library may allow access to the functional diversity of uncultivable microbiota and to the discovery of novel enzymes useful for biotechnological applications. A persisting bottleneck, however, is the lack of methods for large scale production of metagenome-sourced enzymes from genes of unknown origin in the commonly used microbial hosts. To our knowledge, this is the first report on a novel metagenome-sourced enzyme produced in hundreds-of-milligram amount by recovering the protein in the biologically active form from recombinant E. coli inclusion bodies.

More than 3,5 billion people currently lives in cities, a number that is expected to increase further. The densification of cities makes it increasingly difficult for people to access natural areas, at the same time as biological diversity is threatened. In this study, we highlight the importance of urban ecosystem services through social and ecological aspects. This study presents a summary of different evidence-based solutions that contribute with urban ecosystem services. It moreover uses a case of urban development in Slakthusområdet (Meatpacking district), Stockholm, in order to describe contemporary processes that integrate solutions that contribute to urban ecosystem services. The results show that there are challenges in implementing these solutions with respect to political, economic, and market factors. The study thereby identifies how examining authorities and policy demands on a green factor tool enable ecosystem service solutions. By studying ecosystem services as part of a social-ecological system, this study ultimately contributes with knowledge on the execution of multifunctional solutions, which are becoming ever more important with the densification of cities around the globe.

In recent decades, collaboration has become a common policy instrument in public administration, both internationally and in Sweden. Inspired by scholarly literature on collaborative governance, the aim of this study is to analyze the crucial role of public administration in the design and implementation of collaborative governance. Drawing on several years of research on Swedish forest policy and governance, our work is based on extensive empirical material, including 88 semi-structured interviews, observations, written comments from open public consultations and actors, enacted policy documents, open public hearings and a survey. Our results confirm that factors related to process design strongly affect the outputs and outcomes of collaboration in public administration. We assert that public officials should meticulously design and adapt the collaborative process during its initiation and progress, according to the policy problem and actors' incentives and motivations to participate. However, despite good intentions by public officials, the overarching priorities and contextual factors governing the policy area must be set by elected decision makers at an early stage to establish democratic accountability and high levels of policy legitimacy and acceptance. A major implication for public administration is that the increasing use of collaborative governance may be highly inefficient if it is difficult for participants to draft shared objectives and provide intended outputs because of low levels of trust, and different interpretations of knowledge and norms. Finally, in contentious policy areas, such as forest policy, political priorities must sometimes be set by elected decision makers rather than through collaborative processes.

Seagrass meadows, and other submerged vegetated habitats, support a wide range of essential ecological services, but the true extents of these services are in many ways still not quantified. One important tool needed to assess and model many of these services is accurate estimations of the systems´ primary productivity. Such productivity estimations require an understanding of the underwater light field, especially regarding the amount of light that actually reaches the plants' photosynthetic tissue. In this study, we tested a simple practical approach to estimate leaf light exposure, relative to incoming light at the canopy, by attaching light sensitive film at different positions on leaves of Zostera marina, eelgrass, in four seagrass meadows composed of different shoot density and at two different depths. We found that the light reaching the leaves decreased linearly down through the canopy. While the upper parts of the leaves received approximately the same level of light (photosynthetic photon flux density, PPFD) as recorded with a PAR meter at the canopy top, the average light that the seagrass leaves were exposed to varied between 40 and 60% of the light on top of the canopy, with an overall average of 48%. We recommend that actual light interception is measured when assessing or modelling light depending processes in submerged vegetation, but if this is not achievable a rough estimation for vegetation similar to Z. marina would be to use a correction factor of 0.5 to compensate for the reduced light due to leaf orientation and internal shading.

The requirement to review the links between food security, sustainability, and sovereignty - in the provision of food - has increased in an uncertain and unstable environment. Increased local and small-scale food production could, on a national level, make the food system more robust and resilient in the event of disturbances and crises. This study intends to give a broad picture of consumers' attitude, commitment, knowledge and behavior regarding preparedness, food safety and consumption of locally produced food. The study also intends to examine Sweden's national ambition to promote the development of a sustainable, long-term, and competitive food chain and in what way society contributes or can contribute to facilitating and stimulating that type of business. The result shows that there is a strong positive attitude, medium level of knowledge, medium commitment and strong positive behavior among the respondents. The results also show that the attitude towards locally produced food among consumers is positive and can be mainly attributed to the feeling of belonging and the protection of the local living countryside. Regarding the issue of preparedness, there is a clear connection to small-scale and locally produced food production as well as self-sufficiency among the respondents. However, there are major obstacles regarding the price and availability, both in terms of range, opening hours and communication. It is today the most resourceful consumer groups in the society that are able to buy locally produced products and there is a risk of increasing segregation in this. The state could also push to get a definition of locally produced food in place and work with soft control instruments such as, for example, an environmental label. They could also raise the amount for direct procurement within the framework of the EU-directive on public procurement so that the municipalities can buy a greater proportion of locally produced food in their public procurement.

The purpose of this paper is to investigate, based on a comparative study in Sweden, how vertical hydroponic farms can be implemented in urban environments and to identify the challenges with the implementation. The study examines various companies that work with hydroponic farms in Sweden and how the government can help the companies with the implementation of the innovation. Urban hydroponic farms can be a significant solution for solving the growing global population demand for locally grown fresh food, which includes good quality and nutrients. Previous studies, from a sustainability perspective, show that Sweden would benefit in several different ways if more hydroponic farms were implemented in the urban cities. The theory that the report is based on is the implementation theory. With this in mind, the empirics were analyzed from three different found themes; economy, political priorities & local involvement. Empirical data was collected from previous studies as well as from a qualitative methodological approach. This empirical study was based on multiple interviews with companies in the hydroponic farms industry located in Sweden and observations, to be able to analyze the findings based on our chosen theme. In this study we found that the result indicates that there are both advantages and obstacles to the implementation of hydroponic farms in urban areas. On the other hand, the benefits are predominantly from a sustainable development perspective with new innovative solutions, such as solar systems and upgraded district heating and district cooling systems.

Abstract Denial of scientific findings is neither a new nor an unexplored phenomenon. In the area of environmental science and policy though, the research on denial has not been systematically summarized and analyzed. This article reviews 161 scientific articles on environmental and climate science denial published in peer reviewed international journals in the last 25 years and aims to both identify research gaps and enable learning on the phenomenon. Such knowledge is needed for the increasingly important task to provide effective response to science denial, in order to put an end to its influence on environmental policy making. The review, which is based on articles found in the databases Web of Science, Scopus and Philosopher's Index, shows that denial by far is most studied in relation to climate change, with a focus on Anglo-American countries, where this form of denial is most common. Other environmental issues and other geographical areas have received much less scientific attention. While the actors behind climate science denial, their various motives and the characteristics of their operations have been thoroughly described, more comparative research between issues and countries is needed in order to draw reliable conclusions about the factors explaining the peculiarities of denial. This may in turn lay the ground for developing and actually testing the effectiveness and efficiency of strategies to counter environmental science denial. Irrespective of the ambitions of environmental goals, science-based policies are always preferable. The scientific community therefore needs to increase its efforts to dismantle false claims and to disclose the schemes of denialists.

The Ephedra lineage can be traced at least to the Early Cretaceous. Its characteristically polyplicate pollen is well-represented in the fossil record and is frequently used as an indicator of paleoclimate. However, despite previous efforts, knowledge about variation and evolution of ephedroid pollen traits is poor. Here, we document pollen morphology of nearly all extant species of Ephedra, using a combination of scanning electron microscopy (SEM) and light microscopy (LM), and reconstruct ancestral states of key pollen traits. Our results indicate that the ancestral Ephedra pollen type has numerous plicae interspaced by unbranched pseudosulci, while the derived pollen type has branched pseudosulci and (generally) fewer plicae. The derived type is inferred to have evolved independently twice, once along the North American stem branch and once along the Asian stem branch. Pollen of the ancestral type is common in Mesozoic fossil records, especially from the Early Cretaceous, but it is less commonly reported from the Cenozoic. The earliest documentation of the derived pollen type is from the latest Cretaceous, after which it increases strongly in abundance during the Paleogene. The results of the present study have implications for the age of crown group Ephedra as well as for understanding evolution of pollination syndromes in the genus.

With the large increase in transportation over the last decades and the associated negative impacts upon the environment and society, a more sustainable use of transport is a crucial policy issue. This analysis focuses on road freight transport of selected produce (carrots, cabbage, apples and pears) with the aim to appraise the sustainability of road freight transport of these for the Slovene market. To this end, we take into account self-sufficiency, import and export features, transport needs, produce origin and prices differences between domestic and non-domestic produce. The method used for obtaining transported quantities, exported from and imported to the county, was material flow accounts (MFA). Then we undertook an analysis of sustainability of road transport of produce where we considered the country's transport needs. The study finds that road freight transport for selected produce is not sustainable. Recognising the normative dimensions of sustainability, the role of social marketing in this context is explored and suggestions on how to promote more sustainable transport solutions advanced.

In response to the contemporary globalization ofthe economy, food markets are shifting toward differentiation of services and products based on theunique qualities and attributes of the products. Aparadigm called the “quality turn” corresponds to the increasing variety of food services. “Alternative foods”,including organic products or products qualified bytheir origin, and new methods of marketing the sefoods (farmer’s markets, local contracts, etc.) are developingthrough the mainstreaming of innovation. Protected designation of origin (PDO) is a certificationscheme that certifies products by their origin, and is one of several important tools to strengthen the competitiveness of rural areas, especially for smallscalefood processing in rural and less-developed areas in Europe. A PDO provides groups of producers with protection against unfair competition for products whose unique sensory characteristics essentially depend on the local geographic and cultural conditions as well as the local know-how of the productionsite. A PDO certification informs consumers that the product quality and its value depend on the geographic origin of the product. Despite the potential value of PDOs for producers, their use is unevenly distributed throughout the EU. The organization of the qualitycertification systems and corresponding legal provisions vary between countries. France, Italy, and Spainare models for the development of the PDO schemeand have more than 800 PDO-certified products. However, countries such as Sweden, Finland, andDenmark have a much smaller number of products that are certified. In Sweden, several products have applied for a PDO, but only one, Kalix Löjrom, has been certified under the scheme. The reason for this failure is mainly that Sweden’s current customs do not correspond to the rules and traditions used to createthe PDO scheme. To increase the likelihood of successfully obtaining PDOs, Sweden should work to reinvent local knowledge and local food and to recover its traditional food culture.

I och med att hästen tar allt större plats i det peri-urbana landskapet sker det en omstrukturering av både det sociala och det fysiska landskapet. Denna ”hästifiering” omfattar ofta en typ av livsstilsmigration, där den peri-urbana landsbygdens nya invånare och inpendlare (hästägarna) omvandlar miljön. Hästarnas ökade närvaro påverkar nämligen både det privata och det offentliga rummet. Med stöd i över 30 intervjuer konstaterar studien att denna omstrukturering av det peri-urbana landskapet skapar konflikter mellan ”nya” (livsstilsmigranter, hästägare) och de ”gamla” som bott där sedan tidigare (jordbrukare och markägare), vilket kan tolkas som motsättningar mellan grupper med olika ideologier och värderingar där rurala förhållningssätt utmanas av en urban norm. En central aspekt av dessa ”kulturkrockar” är att en urban norm om landskapet som yta för konsumtion och rekreation ofta står i motsatsställning till rurala värderingar och praktiker där landskapet i högre grad utgör en källa till försörjning.