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  • 1.
    Ardila-Ardila, A.
    et al.
    University of Miami/Jackson Memorial Medical Center, USA.
    Goodkin, K.
    University of Miami/Jackson Memorial Medical Center, USA.
    Concha-Bartolini, M.
    University of Miami/Jackson Memorial Medical Center, USA.
    Lecusay-Ruiz, Robert
    University of Miami/Jackson Memorial Medical Center, USA.
    O'Mellan-Fajardo, S.
    University of Miami/Jackson Memorial Medical Center, USA.
    Suarez-Bustamante, P.
    University of Miami/Jackson Memorial Medical Center, USA.
    Molina-Vasquez, R.
    University of Miami/Jackson Memorial Medical Center, USA.
    Lee, D.
    University of Miami/Jackson Memorial Medical Center, USA.
    Chayeb, G.
    University of Miami/Jackson Memorial Medical Center, USA.
    Wilkie, F. L.
    University of Miami/Jackson Memorial Medical Center, USA.
    HUMANS: una batería neuropsicologica para la evaluación de pacientes infectados con VIH-1: [Humans: a neuropsychological battery for evaluating HIV-1 infected patients]2003In: Revista de Neurocirugía, ISSN 1514-3716, Vol. 36, no 8, p. 756-762Article, review/survey (Refereed)
    Abstract [en]

    Objective. To develop a neuropsychological test battery in Spanish for the cognitive evaluation of HIV-1 infected patients. Development. Departing from the suggestions presented by the work group of the National Institute of Mental Health (USA), a neuropsychological assessment battery was developed. It was named HUMANS (HIV/University of Miami Annotated Neuropsychological test battery in Spanish). This battery includes the following domains: 1) attention and speed of processing information, 2) memory, 3) executive function, 4) language, 5) visuospacial/visuoconstructive abilities, and 6) motor abilities. Administration takes about 3-4 hours. The English parallel version of this battery has been successfully used in English for over a decade with HIV-1 infected patients. In the paper the development and adaptation to Spanish language of the HUMANS neuropsychology section is presented Conclusions. HUMANS neuropsychological test battery fulfill the recommendations presented by the workgroup of the National Institute of Mental Health for evaluating HIV-1 infected patients. Studies regarding validity and reliability are still required.

  • 2. Fisher, Linda
    et al.
    Samuelsson, Malin
    Jiang, Yang
    Ramberg, Veronica
    Figueroa, Ricardo
    Södertörn University, School of Life Sciences.
    Hallberg, Einar
    Södertörn University, School of Life Sciences.
    Langel, Ulo
    Iverfeldt, Kerstin
    Targeting cytokine expression in glial cells by cellular delivery of an NF-kappa B decoy2007In: Journal of Molecular Neuroscience, ISSN 0895-8696, E-ISSN 1559-1166, Vol. 31, no 3, p. 209-219Article in journal (Refereed)
    Abstract [en]

    Inhibition of nuclear factor (NF)-kappa B has emerged as an important strategy for design of anti-inflammatory therapies. In neurodegenerative disorders like Alzheimer's disease, inflammatory reactions mediated by glial cells are believed to promote disease progression. Here, we report that uptake of a double-stranded oligonucleotide NF-kappa B decoy in rat primary glial cells is clearly facilitated by noncovalent binding to a cell-penetrating peptide, transportan 10, via a complementary peptide nucleic acid (PNA) sequence. Fluorescently labeled oligonucleotide decoy was detected in the cells within 1 h only when cells were incubated with the decoy in the presence of cell-penetrating peptide. Cellular delivery of the decoy also inhibited effects induced by a neurotoxic fragment of the Alzheimer beta-amyloid peptide in the presence of the inflammatory cytokine interleukin (IL)-1 beta. Pretreatment of the cells with the complex formed by the decoy and the cell-penetrating peptide-PNA resulted in 80% and 50% inhibition of the NF-kappa B binding activity and IL-6 mRNA expression, respectively.

  • 3. Gabriel, J. P.
    et al.
    Mahmood, R.
    Walter, A. M.
    Kyriakatos, A.
    Hauptmann, Giselbert
    Södertörn University, School of Life Sciences. Karolinska Institute.
    Calabrese, R. L.
    El Manira, A.
    Locomotor pattern in the adult zebrafish spinal cord in vitro2008In: Journal of Neurophysiology, ISSN 0022-3077, E-ISSN 1522-1598, Vol. 99, no 1, p. 37-48Article in journal (Refereed)
    Abstract [en]

    The zebrafish is an attractive model system for studying the function of the spinal locomotor network by combining electrophysiological, imaging, and genetic approaches. Thus far, most studies have been focusing on embryonic and larval stages. In this study we have developed an in vitro preparation of the isolated spinal cord from adult zebrafish in which locomotor activity can be induced while the activity of single neurons can be monitored using whole cell recording techniques. Application of NMDA elicited rhythmic locomotor activity that was monitored by recording from muscles or ventral roots in semi-intact or isolated spinal cord preparations, respectively. This rhythmic activity displayed a left-right alternation and a rostrocaudal delay. Blockade of glycinergic synaptic transmission by strychnine switched the alternating activity into synchronous bursting in the left and right sides as well as along the rostrocaudal axis. Whole cell recordings from motoneurons showed that they receive phasic synaptic inputs that were correlated with the locomotor activity recorded in ventral roots. This newly developed in vitro preparation of the adult zebrafish spinal cord will allow examination of the organization of the spinal locomotor network in an adult system to complement studies in zebrafish larvae and new born rodents.

  • 4.
    Svenaeus, Fredrik
    Södertörn University, School of Culture and Education, Centre for Studies in Practical Knowledge.
    Det svänger om hjärnans synapser2020In: Svenska Dagbladet, ISSN 1101-2412, no 2020-11-25Article in journal (Other (popular science, discussion, etc.))
  • 5.
    Tong, Yong-Guang
    et al.
    Södertörn University, School of Life Sciences, Molecular biology.
    Bürglin, Thomas R.
    Södertörn University, School of Life Sciences, Molecular biology.
    Conditions for dye-filling of sensory neurons in Caenorhabditis elegans2010In: Journal of Neuroscience Methods, ISSN 0165-0270, E-ISSN 1872-678X, Vol. 188, no 1, p. 58-61Article in journal (Refereed)
    Abstract [en]

    Dye-filling is a common method used to stain Caenorhabditis elegans ensory neurons in vivo. While the amphids and phasmids are easy to tain, a subset of sensory neurons, the IL2 neurons, are difficult to tain reproducibly. Here we examined the conditions under which the IL2 eurons take up the lipophilic fluorescent dye DiI. We find that IL2 ye-filling depends on salt concentration, but not osmolarity. Low salt rior and during incubation is important for efficient dye uptake. dditional parameters that affect dye-filling are the speed of shaking uring incubation and the addition of detergents. Our modified ye-filling procedure provides a reliable method to distinguish mutant lleles that stain amphids and phasmids, IL2 neurons, or both. An dditional benefit is that it can also stain the excretory duct. The ethod allows genetic screens to be performed to identify mutants that electively affect only one of the sensory structures or the excretory uct.

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