sh.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Mutagenesis of a Dengue virus replicon by introducing stop codons within NS5
Södertörn University, School of Natural Sciences, Technology and Environmental Studies.
Södertörn University, School of Natural Sciences, Technology and Environmental Studies.
2011 (English)Independent thesis Advanced level (degree of Master (One Year)), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Dengue Virus (DENV) is a positive single stranded RNA virus presented in four serotypes, DEN1V, DEN2V, DEN3V and DEN4V. Its structure is composed of three structural proteins, capsid, precursor membrane (prM) and envelope, and seven non structural (NS) proteins, NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5. NS5 has a vital role in the replication of flavivirus. In this project, four different experimental procedures based on standard protocols, were used to create mutations in NS5 of DEN2V. There was no growth in original plates after overnight observation in experiment 1 and in experiment 2, competent cells were streaked on LB ampicillin plates but overgrowth reported in the result after overnight observation. Experience.3 the result of sequencing demonstrated that there was no change in the sequence of NS5 (DEN2V) as compared to the original sequence. Out of all the experimental procedures, optimized mutational results in experiment. 4 i-e., insertion of stop codon TAA and TGA were achieved in NS5 of DEN2V Replicon. It is clear from the sequencing analysis of NS5 of DEN2V Replicon after site directed mutagenesis that two stop codons (TAA & TGA) are inserted instead of AAA & AGA in the original SN5 sequence of DEN2V.

Place, publisher, year, edition, pages
2011. , 21 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:sh:diva-28645OAI: oai:DiVA.org:sh-28645DiVA: diva2:865269
Subject / course
Molecular Biology
Uppsok
Medicine
Supervisors
Available from: 2015-10-28 Created: 2015-10-27 Last updated: 2016-07-06Bibliographically approved

Open Access in DiVA

fulltext(486 kB)128 downloads
File information
File name FULLTEXT01.pdfFile size 486 kBChecksum SHA-512
0d9a769d7f96b67cf91c4232a246965261f245100e79ba85c98fd5c268378a142c4c6cd8b318296b88d5bd241040c47dfddbb6b0783d5947634c86a9ed598888
Type fulltextMimetype application/pdf

By organisation
School of Natural Sciences, Technology and Environmental Studies
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 128 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 130 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf