sh.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
Developmental exposure of zebrafish (Daniorerio) to 17α-Ethinylestradiol affects non-reproductive behavior and fertility as adults, and increases anxiety in unexposed progeny
Södertörn University, School of Natural Sciences, Technology and Environmental Studies, Biology. Örebro University.
Södertörn University, School of Natural Sciences, Technology and Environmental Studies, Biology. Örebro University.
Södertörn University, School of Natural Sciences, Technology and Environmental Studies, Environmental Science.
Södertörn University, School of Natural Sciences, Technology and Environmental Studies, Biology.
Show others and affiliations
2015 (English)In: Hormones and Behavior, ISSN 0018-506X, E-ISSN 1095-6867, Vol. 73, 30-38 p.Article in journal (Refereed) Published
Abstract [en]

Exposure to estrogenic endocrine disruptors (EDCs) during of development affects fertility, reproductive and non-reproductive behavior in mammals and fish. These effects can also be transferred to coming generations. In fish, the effects of developmental EDC exposure on non-reproductive behavior is less well studied. Here, we analyze the effects of 17α-Ethinylestradiol (EE2) on anxiety, shoaling behavior and fertility in zebrafish after developmental treatment and remediation in clean water until adulthood. Zebrafish embryos were exposed from day 1 to day 80 post fertilization to actual concentrations of 1.2 and 1.6ng/L EE2. After remediation for 82days non-reproductive behavior and fertilization success were analyzed in both sexes. Males and females from the 1.2ng/L group, as well as control males and females, were bred, and behavior of the untreated F1 offspring was tested as adults. Developmental treatment with 1.2 and 1.6ng/L EE2 significantly increased anxiety in the Novel Tank test and increased shoaling intensity in both sexes. Fertilization success was significantly reduced by EE2 in both sexes when mated with untreated fish of opposite sex. Progeny of fish treated with 1.2ng/L EE2 showed increased anxiety in the Novel tank test and increased light avoidance in the Scototaxis test compared to control offspring. In conclusion, developmental exposure of zebrafish to low doses of EE2 resulted in persistent changes in behavior and fertility. The behavior of unexposed progeny were affected by their parents' exposure, which might suggest transgenerational effects.

Place, publisher, year, edition, pages
2015. Vol. 73, 30-38 p.
Keyword [en]
17α-Ethinylestradiol; Anxiety; Developmental exposure; Endocrine disruptors; F1 effects; Fertility; Neuroendocrinology; Social behavior; Stress behavior; Zebrafish
National Category
Biological Sciences Environmental Sciences
Research subject
Environmental Studies; Baltic and East European studies
Identifiers
URN: urn:nbn:se:sh:diva-27795DOI: 10.1016/j.yhbeh.2015.05.014ISI: 000360251800005PubMedID: 26072466ScopusID: 2-s2.0-84934983120Local ID: 1556/42/2011OAI: oai:DiVA.org:sh-27795DiVA: diva2:823634
Projects
Endocrine disruption in fish
Funder
The Foundation for Baltic and East European Studies, 1742/42/2008The Foundation for Baltic and East European Studies, 1556/42/2011
Available from: 2015-06-18 Created: 2015-06-18 Last updated: 2016-10-04Bibliographically approved
In thesis
1. Fishy behavior: persistent effects of early-life exposure to 17α-ethinylestradiol
Open this publication in new window or tab >>Fishy behavior: persistent effects of early-life exposure to 17α-ethinylestradiol
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The synthetic estrogen 17α-ethinylestradiol (EE2) is an endocrine disrupting chemical (EDC) of concern due to its persistent nature and widespread presence in the aquatic environment. In mammals, effects of developmental EDC exposure on reproduction and behavior not only persist to adulthood after discontinued exposure, but are also inherited by several consecutive unexposed generations. The results presented in this thesis demonstrate that non-reproductive behavior in fish is highly sensitive to the influence of EE2 during development and the effects do not appear to be restored after a long recovery period in clean water. We have shown that exposure to low doses of EE2 during development results in increased anxiety in two fish species (zebrafish and guppy) and their offspring. We have also demonstrated that the effects of EE2 on anxiety are apparent in both sexes and are transgenerationally transmitted to two consecutive generations of unexposed offspring in the guppy. In order to investigate the possible biological mechanisms of the observed persistent effects on non-reproductive behavior, we also performed an RNA sequencing analysis of the whole-brain transcriptome in developmentally exposed zebrafish after remediation in clean water until adulthood. Differential expression of 33 genes in males and 62 genes in females were observed as a result of EE2 exposure, with only one gene affected in both sexes. Functional analysis revealed cholesterol biosynthesis and circadian rhythm to be the top two affected pathways in males and females, respectively. Both pathways have previously been implicated in anxiety behavior and represent possible candidates connecting the transcriptome alterations to the observed behavioral phenotype. The study represents an initial survey of the fish brain transcriptome by means of RNA sequencing after long-term recovery from developmental exposure to an estrogenic compound.

Place, publisher, year, edition, pages
Örebro: Örebro university, 2015. 62 p.
Series
Örebro Studies in Biology, ISSN 1650-8793 ; 9Södertörn doctoral dissertations, ISSN 1652-7399 ; 110
Keyword
Endocrine disruptors, anxiety, stress behavior, transgenerational effects, 17α-ethinylestradiol, developmental exposure, social behavior, fish
National Category
Biological Sciences
Research subject
Biology
Identifiers
urn:nbn:se:sh:diva-28426 (URN)978-91-7529-091-1 (ISBN)
Public defence
2015-10-05, Hörsalen, Musikhögskolan, Örebro, 13:00 (English)
Opponent
Supervisors
Available from: 2015-10-01 Created: 2015-10-01 Last updated: 2015-10-01Bibliographically approved
2. Hitting the mark: Studies of alterations in behaviour and fertility in ethinyl estradiol-exposed zebrafish and search related biomarkers
Open this publication in new window or tab >>Hitting the mark: Studies of alterations in behaviour and fertility in ethinyl estradiol-exposed zebrafish and search related biomarkers
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In this thesis, we have analysed the effects of EE2 on non-reproductive behaviours and fertility. We have showed that two doses of EE2 in male adult short-term exposures evokes opposite behaviours in the novel tank test. A lower dose induced increased bottom-dwelling, a sign of increased anxiety and a higher dose increased surface-dwelling, which would likely expose themselves to predation in a natural environment. Increased shoaling was observed in both exposures, possibly affecting feeding and reproduction opportunities. Fertility analysis of these fish demonstrated a complete inhibition of spawning in the highest dose group. To investigate mechanisms behind the spawning failure, we examined expression levels of genes involved in zebrafish sex differentiation and maintenance of gonadal function. We found downregulated transcription levels of male-predominant genes, suggesting a demasculinization of the testes contributing to functional sterility in these fish. We have demonstrated that non-reproductive behaviour in zebrafish is highly sensitive to EE2 exposure during development. After exposing male and female zebrafish to low doses of EE2 followed by remediation in clean water until adulthood, the fish displayed increased anxiety and shoaling behaviour, demonstrating persistent effects of EE2. Furthermore, behavioural effects were transferred to their progeny. Decreased fertilisation success of the developmentally exposed fish was observed in both sexes when mated to untreated animals of the opposite sex. These fertility effects persisted although the fish had a long remediation period, implying likely reduced fitness of fish populations in aquatic environments. Based on our findings on non-reproductive behaviours and fertility, we performed RNAsequencing analysis of the brain and testes in order to investigate possible biological mechanisms behind the persistent effects. There is a need for biomarkers allowing detection of both reversible and irreversible effects in animals exposed to estrogenic substances, hopefully contributing to better risk assessments for EDCs. Results from RNA-sequencing would serve as a basis for continued studies in pursuit of potential biomarkers.

Place, publisher, year, edition, pages
Örebro: Örebro university, 2016. 55 p.
Series
Örebro Studies in Biology, ISSN 1650-8793 ; 10Södertörn doctoral dissertations, ISSN 1652-7399 ; 115
Keyword
Endocrine disrupting compounds, 17α-ethinylestradiol, fertility, anxiety, behaviour, zebrafish, biomarkers, stress
National Category
Other Biological Topics
Identifiers
urn:nbn:se:sh:diva-29440 (URN)978-91-7529-115-4 (ISBN)
Public defence
2016-02-25, Sal MA636, Alfred Nobels allé 7, Huddinge, 13:00 (English)
Opponent
Supervisors
Funder
The Foundation for Baltic and East European Studies
Available from: 2016-02-04 Created: 2016-02-04 Last updated: 2016-02-04Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Volkova, KristinaCaspillo, Nasim ReyhanianPorseryd, ToveHallgren, StefanDinnétz, PatrikPorsch-Hällström, Inger
By organisation
BiologyEnvironmental Science
In the same journal
Hormones and Behavior
Biological SciencesEnvironmental Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 363 hits
ReferencesLink to record
Permanent link

Direct link