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Isolation of the promoters of Atlantic salmon MHCII genes
Södertörn University, Avdelning Naturvetenskap. Norwegian Sch. of Veterinary Science, Oslo, Norway .
2003 (English)In: Marine Biotechnology, ISSN 1436-2228, E-ISSN 1436-2236, Vol. 5, no 3, 253-260 p.Article in journal (Refereed) Published
Abstract [en]

The major histocompatibility complex class II (MHCII) has a central role in the immune response of vertebrates with its function of presenting antigenic peptides to the T-cell receptors. We have isolated the promoters and intron 1 of MHCIIα and MHCIIβ genes of Atlantic salmon. To isolate these promoters, we constructed an Atlantic salmon (Salmo salar) promoter finder kit (analogous to the commercially available "human promoter finder kit"). By nucleotide sequence alignment of known MHCII promoter regions, we identified the 3 conserved regulatory X, X2, and Y boxes in the salmon promoters. The W box was not found. In contrast, a salmon-specific putative W box was identified. Both of the isolated Atlantic salmon MHCIIα and β promoters (included in patent applications by Genomar A/S, Oslo, Norway) were found to be functional since they both gave positive yellow fluorescence protein signal when inserted as promoters in the pEYFP-1 reporter plasmid and transfected into the salmon head kidney cell line (SHK-1).

Place, publisher, year, edition, pages
2003. Vol. 5, no 3, 253-260 p.
Keyword [en]
Major histocompatibility complex, MHC promoter, Salmo salar, SHK-1 cells, Transfection, salmonid, Animals, Bacterial Proteins, Base Sequence, DNA Primers, Genes, MHC Class II, Luminescent Proteins, Microscopy, Fluorescence, Molecular Sequence Data, Promoter Regions (Genetics), Sequence Alignment, Salmo, Salmonidae, Vertebrata
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:sh:diva-23218DOI: 10.1007/s10126-002-0063-4ISI: 000184184300006Scopus ID: 2-s2.0-0042976108OAI: oai:DiVA.org:sh-23218DiVA: diva2:715602
Available from: 2014-05-05 Created: 2014-04-16 Last updated: 2014-05-05Bibliographically approved

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Lundin, Maria
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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf