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Proteomic analysis of the Drosophila larval hemolymph clot
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2004 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 279, no 50, 52033-52041 p.Article in journal (Refereed) Published
Abstract [en]

Components of the insect clot, an extremely rapid forming and critical part of insect immunity, are just beginning to be identified (1). Here we present a proteomic comparison of larval hemolymph before and after clotting to learn more about this process. This approach was supplemented by the identification of substrates for the enzyme transglutaminase, which plays a role in both vertebrate blood clotting (as factor XIIIa) and hemolymph coagulation in arthropods. Hemolymph proteins present in lower amounts after clotting include CG8502 (a protein with a mucin-type domain and a domain with similarity to cuticular components), CG11313 (a protein with similarity to prophenoloxidase-activating proteases), and two phenoloxidases, lipophorin, a secreted gelsolin, and CG15825, which had previously been isolated from clots (2). Proteins whose levels increase after clotting include a ferritin-subunit and two members of the immunoglobulin family with a high similarity to the small immunoglobulin-like molecules involved in mammalian innate immunity. Our results correlate with findings from another study of coagulation (2) that involved a different experimental approach. Proteomics allows the isolation of novel candidate clotting factors, leading to a more complete picture of clotting. In addition, our two-dimensional protein map of cell-free Drosophila hemolymph includes many additional proteins that were not found in studies performed on whole hemolymph.

Place, publisher, year, edition, pages
2004. Vol. 279, no 50, 52033-52041 p.
Keyword [en]
Enzymes, Immunology, Proteins, Substrates, Clotting, Hemolymph, Insect clot, Insect immunity, Biochemistry, blood clotting factor 13a, ferritin, gelsolin, immunoglobulin, lipophorin, lymph protein, protein cg11313, protein cg15825, protein cg8502, protein glutamine gamma glutamyltransferase, protein subunit, unclassified drug, article, blood clotting, comparative study, controlled study, Drosophila, enzyme substrate, larva, nonhuman, priority journal, protein analysis, protein content, protein depletion, protein isolation, proteomics, sequence analysis, Amino Acid Sequence, Animals, Blood Proteins, Drosophila Proteins, Electrophoresis, Gel, Two-Dimensional, Genes, Insect, Molecular Sequence Data, Sequence Homology, Amino Acid, Substrate Specificity, Transglutaminases, Arachnida, Arthropoda, Hexapoda, Insecta, Mammalia, Vertebrata
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Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:sh:diva-23108DOI: 10.1074/jbc.M408220200ISI: 000225493400040ScopusID: 2-s2.0-10644295420OAI: oai:DiVA.org:sh-23108DiVA: diva2:713784
Available from: 2014-04-24 Created: 2014-04-16 Last updated: 2014-04-24Bibliographically approved

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