DNA damage caused by etoposide and γ-irradiation induces gene conversion of the MHC in a mouse non-germline testis cell line
1999 (English)In: Mutation research, ISSN 0027-5107, E-ISSN 1873-135X, Vol. 423, no 1-2, 155-169 p.Article in journal (Refereed) Published
We have explored the effects of γ-irradiation and etoposide on the gene conversion frequency between the endogenous major histocompatibility complex class II genes Abk and Ebd in a mouse testis cell line of non-germline origin with a polymerase chain reaction assay. Both γ-rays and etoposide were shown to increase the gene conversion frequency with up to 15-fold compared to untreated cells. Etoposide, which is an agent that stabilise a cleavable complex between DNA and DNA topoisomerase II, shows an increased induction of gene conversion events with increased dose of etoposide. Cells treated with γ-rays, which induce strand breaks, had an increased gene conversion frequency when they were subjected to low doses of irradiation, but increasing doses of irradiation did not lead to an increase of gene conversion events, which might reflect differences in the repair process depending on the extent and nature of the DNA damage. These results where DNA damage was shown to be able to induce gene conversion of endogenous genes in mouse testis cells suggests that the DNA repair system could be involved in the molecular genetic mechanism that results in gene conversion in higher eukaryotes like mammals.
Place, publisher, year, edition, pages
1999. Vol. 423, no 1-2, 155-169 p.
γ-Irradiation, DNA damage, Etoposide, Gene conversion, MHC, Mouse, carcinogen, DNA topoisomerase (ATP hydrolysing), major histocompatibility antigen class 2, animal cell, article, controlled study, DNA repair, eukaryote, gamma irradiation, germ cell, immunogenetics, male, mammal, nonhuman, polymerase chain reaction, priority journal, testis cell, Amino Acid Sequence, Animals, Base Sequence, Cells, Cultured, Crosses, Genetic, DNA Mutational Analysis, Dose-Response Relationship, Drug, Dose-Response Relationship, Radiation, Gamma Rays, Genes, MHC Class II, Mice, Mice, Inbred BALB C, Mice, Inbred C3H, Molecular Sequence Data, Sequence Alignment, Testis, Animalia, Eukaryota, Mammalia
IdentifiersURN: urn:nbn:se:sh:diva-22928DOI: 10.1016/S0027-5107(98)00239-5PubMedID: 10029693ScopusID: 2-s2.0-0033601827OAI: oai:DiVA.org:sh-22928DiVA: diva2:711882