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Differential MHC expression requirements for positive selection of separate TCR Vb families
Södertörn University, Avdelning Naturvetenskap. Karolinska Institute.
1999 (English)In: Immunogenetics, ISSN 0093-7711, E-ISSN 1432-1211, Vol. 49, no 1, 1-6 p.Article in journal (Refereed) Published
Abstract [en]

Positive selection has been proposed to be involved in protection from diabetes. We examined positive selection by fluorescence-activated cell sorter analyses in thymocytes of protected and susceptible E-transgenic and non-transgenic NOD mice. Three Vb families showed positive selection in E-transgenic mice. Vb6+CD4+ and Vb10+CD4+ thymocytes were found at higher frequencies in both protected NOD-Ea and susceptible NOD-DY mice. The increased frequencies of Vb13+CD8+ thymocytes were found in protected NOD-Ea mice only, and not in susceptible NOD-DY transgenic mice. These three Vb families were further examined in bone-marrow chimeras between NOD-Ea and non-transgenic NOD mice, where we could examine the contribution of E-expressing bone-marrow-derived cells in positive selection. We find that NOD-Ea→NOD-Ea chimeras have an increased positive selection of Vb13+CD8+ cells and that positive selection is more efficient when both thymic epithelium and bone-marrow-derived cells express the E molecule. This was also seen for Vb6+CD4+ cells. However, for Vb6, bone-marrow-derived cells alone were also capable of positive selection. Positive selection of Vb10+CD4+ cells was restricted to E-expressing thymic epithelium only. For Vb13+CD8+ cells, we found that positive selection is most efficient with E-expression on both thymic epithelium and bone-marrow-derived cells, although positive selection also occurs with E-positive epithelium only. For Vb6+CD4+ cells, the dominating selecting cells are bone-marrow-derived cells, and Vb10+CD4+ cells seem to be selected exclusively by the thymic epithelium. Thus, the conditions for positive selection seem to vary considerably between different Vb families.

Place, publisher, year, edition, pages
1999. Vol. 49, no 1, 1-6 p.
Keyword [en]
Bone marrow, Postive selection, Thymic epithelium, Vb-NOD, cd4 antigen, cd8 antigen, major histocompatibility antigen, t lymphocyte receptor, animal cell, animal experiment, antigen expression, article, bone marrow cell, cell selection, chimera, controlled study, diabetes mellitus, fluorescence activated cell sorter, genetic susceptibility, mouse, nonhuman, priority journal, thymocyte, transgenic mouse, Animals, Autoimmune Diseases, Bone Marrow Cells, Bone Marrow Transplantation, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Clonal Deletion, Diabetes Mellitus, Type 1, Female, Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, Histocompatibility Antigens Class II, Mice, Mice, Inbred C57BL, Mice, Inbred NOD, Mice, Transgenic, Radiation Chimera, Receptors, Antigen, T-Cell, alpha-beta, T-Lymphocyte Subsets, Thymus Gland, Animalia, Mus musculus
National Category
Immunology
Identifiers
URN: urn:nbn:se:sh:diva-22927DOI: 10.1007/s002510050457ISI: 000077297800001PubMedID: 9811963Scopus ID: 2-s2.0-0032953289OAI: oai:DiVA.org:sh-22927DiVA: diva2:710631
Available from: 2014-04-07 Created: 2014-03-28 Last updated: 2014-04-07Bibliographically approved

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