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Radiation and hypothermia: Changes in DNA supercoiling in human diploid fibroblasts
Södertörn University, Avdelning Naturvetenskap. Karolinska Institute.
1999 (English)In: Anticancer Research, ISSN 0250-7005, Vol. 19, no 6B, 5307-5311 p.Article in journal (Refereed) Published
Abstract [en]

The influence of hypothermia (2 degrees, 15 degrees and 28 degrees C) ripen the effect of X-irradiation on chromatin from human diploid fibroblast cells (AG1518) was studied using the fluorescent halo assay. Rewinding of supercoils was inhibited in a dose-dependent manner when cells were irradiated with 4, 8 or 16 Gy. This inhibition of rewinding was reduced when cells were irradiated at subnormal temperatures compared with cells irradiated at 37 degrees C. One hour's preincubation at low temperature did nor influence rewinding. When AG1518 cells were irradiated at 37 degrees C in the presence of the radical scavenger DMSO (0.5 M), the radiation-induced damage was reduced. No additional protection of DMSO in hypothermic cells (2 degrees C) was found, possibly indicating that OH-radical-mediated effects al-e more temperature dependent. These results are similar to those recently found for the malignant MCF-7 cell line.

Place, publisher, year, edition, pages
1999. Vol. 19, no 6B, 5307-5311 p.
Keyword [en]
radiation, temperature, DNA supercoiling, nucleoid, DMSO
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:sh:diva-22306ISI: 000085317300061PubMedID: 10697553ScopusID: 2-s2.0-0033376739OAI: oai:DiVA.org:sh-22306DiVA: diva2:697589
Available from: 2014-02-18 Created: 2014-02-18 Last updated: 2014-02-18Bibliographically approved

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