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RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe
Södertörn University, School of Natural Sciences, Technology and Environmental Studies, Biology. Karolinska instiutet.
Karolinska instiutet.
Karolinska instiutet.
Södertörn University, School of Natural Sciences, Technology and Environmental Studies, Biology.
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2014 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 444, no 2, p. 254-259Article in journal (Other academic) Published
Abstract [en]

RNA interference (RNAi) is a gene silencing mechanism conserved from fungi to mammals. Small interfering RNAs are products and mediators of the RNAi pathway and act as specificity factors in recruiting effector complexes. The Schizosaccharomyces pombe genome encodes one of each of the core RNAi proteins, Dicer, Argonaute and RNA-dependent RNA polymerase (dcr1, ago1, rdp1). Even though the function of RNAi in heterochromatin assembly in S. pombe is established, its role in controlling gene expression is elusive. Here, we report the identification of small RNAs mapped anti-sense to protein coding genes in fission yeast. We demonstrate that these genes are up-regulated at the protein level in RNAi mutants, while their mRNA levels are not significantly changed. We show that the repression by RNAi is not a result of heterochromatin formation. Thus, we conclude that RNAi is involved in post-transcriptional gene silencing in S. pombe.

Place, publisher, year, edition, pages
2014. Vol. 444, no 2, p. 254-259
Keywords [en]
Fission yeast, PTGS, RNAi
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:sh:diva-22232DOI: 10.1016/j.bbrc.2014.01.057ISI: 000331923500025PubMedID: 24462781Scopus ID: 2-s2.0-84892926000OAI: oai:DiVA.org:sh-22232DiVA, id: diva2:696634
Funder
The Foundation for Baltic and East European Studies, 300501Swedish Cancer Society, CAN-2009Swedish Research Council, VR-NT-2007-4722Available from: 2014-02-14 Created: 2014-02-14 Last updated: 2018-07-20Bibliographically approved

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Smialowska, AgataKylsten, PerSwoboda, PeterEkwall, Karl

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CiteExportLink to record
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Citation style
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