c-Myc induces changes in higher order rDNA structure on stimulation of quiescent cells
2009 (English)In: Oncogene, ISSN 0950-9232, E-ISSN 1476-5594, Vol. 28, no 16, 1833-1842 p.Article in journal (Refereed) Published
c-Myc is an oncogenic transcription factor capable of activating transcription by all three nuclear RNA polymerases, thus acting as a high-level coordinator of protein synthesis capacity and cell growth rate. c-Myc recruits RNA polymerase I-related transcription factors to the rDNA when quiescent cells are stimulated to re-enter the cell cycle. Using a model system of cell lines with variable c-Myc status, we show that on stimulation c-Myc rapidly induces gene loop structures in rDNA chromatin that juxtapose upstream and downstream rDNA sequences. c-Myc activation is both necessary and sufficient for this change in rDNA chromatin conformation. c-Myc activation induces association of TTF-1 with the rDNA, and c-Myc is physically associated with induced rDNA gene loops. The origins of two or more rDNA gene loops are closely juxtaposed, suggesting the possibility that c-Myc induces nucleolar chromatin hubs. Induction of rDNA gene loops may be an early step in the reprogramming of quiescent cells as they re-enter the growth cycle.
Place, publisher, year, edition, pages
2009. Vol. 28, no 16, 1833-1842 p.
c-Myc, nucleolus, transcription, ribosome biogenesis, RNA polymerase I
Biochemistry and Molecular Biology Cell Biology
IdentifiersURN: urn:nbn:se:sh:diva-17673DOI: 10.1038/onc.2009.21ISI: 000265640500002PubMedID: 19270725ScopusID: 2-s2.0-67349121053OAI: oai:DiVA.org:sh-17673DiVA: diva2:577178