Selection of cefoxitin-resistant Bacteroides thetaiotaomicron mutants and mechanisms involved in beta-lactam resistance
2002 (English)In: Clinical Infectious Diseases, ISSN 1058-4838, Vol. 35, S47-S53 p.Article in journal (Refereed) Published
The beta-lactam antibiotics are the most widely used of all the groups of antimicrobials, but beta-lactam resistance is increasingly common among members of the Bacteroides fragilis group. Three major mechanisms are involved in beta-lactam resistance, and they act together in certain instances. In the present study, 2 resistant mutants (238m and 1186m) of Bacteroides thetaiotaomicron, obtained from clinical isolates (238 and 1186) by selection with increasing concentrations of cefoxitin, showed decreased susceptibilities to cefoxitin and other beta-lactam antibiotics. Alterations in both penicillin-binding proteins (PBPs) and outer-membrane proteins (OMPs) were observed in the mutants in comparison with their parent strains. The similar alteration in OMPs was also observed in clinical isolates. In conclusion, the beta-lactam-resistant mutants of B. thetaiotaomicron with deficiency in both PBPs and OMPs can be selected for by exposure to cefoxitin, and several mechanisms are involved in the beta-lactam resistance in the strains investigated.
Place, publisher, year, edition, pages
2002. Vol. 35, S47-S53 p.
IdentifiersURN: urn:nbn:se:sh:diva-17666DOI: 10.1086/341920ISI: 000177533400008PubMedID: 12173108OAI: oai:DiVA.org:sh-17666DiVA: diva2:577053
Anaerobe Odyssey Symposium held in honor of Sydney M Finegolds 80th Birthday, AUG 12, 2001, LOS ANGELES, CALIFORNIA