sh.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
Cloning of a plastidic rye (Secale cereale) beta-glucosidase cDNA and its expression in Escherichia coli
Södertörn University, Avdelning Naturvetenskap.
2003 (English)In: Physiologia Plantarum: An International Journal for Plant Biology, ISSN 0031-9317, E-ISSN 1399-3054, Vol. 118, no 3, 337-345 p.Article in journal (Refereed) Published
Abstract [en]

The cDNA for a beta-glucosidase (EC3.2.1.21) was isolated from rye (Secale cereale, cv Motto) and the sequence corresponding to the mature protein cloned into pET21a expression vector and used for transformation of Escherichia coli. The recombinant beta-glucosidase expressed in E. coli was recognized by antibodies to maize beta-glucosidase and exhibited the same kinetic properties on the endogenous substrates hydroxamic acid glucosides and artificial substrates as the native enzyme purified from rye. The enzyme monomer had an apparent molecular weight of about 67 kDa. The isolated cDNA was analysed with web-based chloroplast targeting prediction programs. The programs predicted a chloroplast targeting peptide with a cleavage site between amino acid 49 and 50. Sequence alignment of the plastidic rye beta-glucosidase showed that the putative sites for substrate specificity of maize Glu1, W378 and F198 (F197) are conserved in the rye enzyme, whereas F205, F466 and A467 of maize Glu1 are exchanged for histidine, glycine and serine, respectively, in rye. The plastidic beta-glucosidase is expressed in all plant parts and the highest levels were found in the coleoptile and mesocotyl.

Place, publisher, year, edition, pages
2003. Vol. 118, no 3, 337-345 p.
National Category
Botany
Identifiers
URN: urn:nbn:se:sh:diva-17367DOI: 10.1034/j.1399-3054.2003.00118.xISI: 000183604600005ScopusID: 2-s2.0-0038688699OAI: oai:DiVA.org:sh-17367DiVA: diva2:571330
Available from: 2012-11-22 Created: 2012-11-19 Last updated: 2014-04-17Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Jonsson, Lisbeth M V
By organisation
Avdelning Naturvetenskap
In the same journal
Physiologia Plantarum: An International Journal for Plant Biology
Botany

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 38 hits
ReferencesLink to record
Permanent link

Direct link