sh.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
Characterization of MYR1, a dosage suppressor of YPT6 and RIC1 deficient mutants
Södertörn University, School of Life Sciences, Biology.
Södertörn University, School of Life Sciences, Biology.
Södertörn University, School of Life Sciences, Biology.
Show others and affiliations
2008 (English)In: Current Genetics, ISSN 0172-8083, E-ISSN 1432-0983, Vol. 53, no 4, 235-247 p.Article in journal (Refereed) Published
Abstract [en]

Membrane traffic is tightly regulated and the Rab protein family of small GTPases plays a central role in this regulation. One member of this family is the Saccharomyces cerevisae protein Ypt6. To search for new genes interacting with Ypt6-related pathways, we performed a genetic screen for high copy suppressors of ypt6 Delta temperature sensitivity at 35 degrees C. Among the suppressors, MYR1 was also able to suppress the temperature sensitive mutant lacking Ric1, a subunit of the Ypt6 guanine exchanging factor complex Ric1/Rgp1. Myr1 is characterized by a coiled coil region and a GYF domain, a protein module binding proline-rich sequences. Myr1 is able to bind membranes but is also associated with larger structures insoluble in Triton X-100. By immunofluorescence, Myr1 shows a network-like pattern as well as small foci. Overexpression of Myr1 influences nuclear envelope morphology and high levels are lethal. This lethality is rescued when the N-terminal region, containing the GYF domain, is deleted. The transcription profile of a myr1 Delta strain shows effects on genes involved in nuclear migration, Ras signalling and transcription. Taken together, these results suggest that Myr1 is a novel factor linked to the secretory pathway and important cellular regulatory mechanisms.

Place, publisher, year, edition, pages
2008. Vol. 53, no 4, 235-247 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:sh:diva-17359DOI: 10.1007/s00294-008-0183-0ISI: 000254182700005ScopusID: 2-s2.0-41149157331OAI: oai:DiVA.org:sh-17359DiVA: diva2:570788
Available from: 2012-11-20 Created: 2012-11-19 Last updated: 2016-10-10Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Georgiev, AlexanderBerrez, Jean-MarcMutvei, Ann
By organisation
Biology
In the same journal
Current Genetics
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 95 hits
ReferencesLink to record
Permanent link

Direct link