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Cloning and expression of a putative cyclodextrin glucosyltransferase from the symbiotically competent cyanobacterium Nostoc sp PCC 9229
Department of Biology and Environmental Science, University of Kalmar.
Department of Botany, Stockholm University.
Department of Biology and Environmental Science, University of Kalmar.
2003 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 219, no 2, 181-185 p.Article in journal (Refereed) Published
Abstract [en]

A polymerase chain reaction-based method was used to isolate a Nostoc sp. PCC 9229 cDNA from infected glands of Gunnera chilensis. The complete gene sequence was isolated from a genomic Nostoc sp. PCC 9229 library. Sequence analysis showed 84% amino acid similarity to a putative cyclodextrin glycosyltransferase from Nostoc sp. PCC 7120 and the gene was therefore termed cgt. Southern blot revealed that the cgt gene was present in symbiotically competent cyanobacteria. The cgt gene was expressed in free-living nitrogen-fixing cultures in light or in darkness when supplemented with fructose. This is the first expression analysis of a cgt gene from a cyanobacterium. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Place, publisher, year, edition, pages
2003. Vol. 219, no 2, 181-185 p.
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:sh:diva-17124DOI: 10.1016/S0378-1097(02)1204-1ISI: 000181567500005OAI: oai:DiVA.org:sh-17124DiVA: diva2:558066
Funder
Swedish Research Council
Available from: 2012-10-01 Created: 2012-10-01 Last updated: 2012-10-01Bibliographically approved
In thesis
1. Heterocystous cyanobacteria living within symbiosomes: genetic and physiological aspects
Open this publication in new window or tab >>Heterocystous cyanobacteria living within symbiosomes: genetic and physiological aspects
2005 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Kalmar: Högskolan i Kalmar, 2005. 30 p.
Series
Dissertation series / University of Kalmar, Faculty of Natural Science, ISSN 1650-2779 ; 20
Keyword
Microbiology, cyanobacteria, plants, symbiosis, nitrogen-fixation
National Category
Natural Sciences
Identifiers
urn:nbn:se:sh:diva-17116 (URN)91-89584-42-2 (ISBN)
Public defence
(English)
Opponent
Supervisors
Funder
Swedish Research Council
Available from: 2012-10-01 Created: 2012-10-01 Last updated: 2012-10-01Bibliographically approved

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CiteExportLink to record
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  • apa
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