Change search
CiteExportLink to record
Permanent link

Direct link
Monitoring population size, activity, and distribution of gfp-luxAB-tagged Pseudomonas fluorescens SBW25 during colonization of wheat
Södertörn University, Avdelning Naturvetenskap.
2001 (English)In: Microbial Ecology, ISSN 0095-3628, E-ISSN 1432-184X, Vol. 41, no 4, 290-300 p.Article in journal (Refereed) Published
Abstract [en]

Increasingly, focus has been directed towards the use of microorganisms as biological control agents to combat fungal disease, as an alternative to chemical fungicides. Pseudomonas fluorescens SBW25 is one bacterial strain that has been demonstrated to promote plant growth by biocontrol of pathogenic fungi. To understand the mode of action of this bacterium, information regarding its localization and metabolic activity on plants is important. In this study, a gfp/luxAB-tagged derivative of P. fluorescens SBW25, expressing the green fluorescent protein (GFP) and bacterial luciferase, was monitored during colonization of wheat starting from seed inoculation. Since bacterial luciferase is dependent on cellular energy reserves for phenotypic expression, metabolically active cells were detected using this marker. In contrast, the stable GFP fluorescence phenotype was used to detect the cells independently of their metabolic status. The combination of these two markers enabled P. fluorescens SBW25 cells to be monitored on wheat plants to determine their specific location and metabolic activity. Studies on homogenized wheat plant parts demonstrated that the seed was the preferred location of P. fluorescens SBW25 during the 65-day time period studied, but the leaves and roots were also colonized. Interestingly, the bacteria were also found to be metabolically active on all plant parts examined. In situ localization of P. fluorescens SBW25 using a combination of different microscopic techniques confirmed the preference for the cells to colonize specific regions of the seed. We speculate that the colonization pattern of P. fluorescens SBW25 can be linked to the mechanism of protection of plants from fungal infection.

Place, publisher, year, edition, pages
2001. Vol. 41, no 4, 290-300 p.
National Category
Ecology Microbiology
URN: urn:nbn:se:sh:diva-15856DOI: 10.1007/s002480000047ISI: 000169237900002OAI: diva2:509245
Available from: 2012-03-12 Created: 2012-03-09 Last updated: 2012-03-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Jansson, Janet
By organisation
Avdelning Naturvetenskap
In the same journal
Microbial Ecology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 49 hits
CiteExportLink to record
Permanent link

Direct link