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Sequential degradation of proteins from the nuclear envelope during apoptosis
Södertörn University, Avdelning Naturvetenskap.
Södertörn University, Avdelning Naturvetenskap.
Södertörn University, Avdelning Naturvetenskap.
2001 (English)In: Journal of Cell Science, ISSN 0021-9533, E-ISSN 1477-9137, Vol. 114, no 20, 3643-3653 p.Article in journal (Refereed) Published
Abstract [en]

We have produced new antibodies specific for the integral pore membrane protein POM121. Using these antibodies we show that during apoptosis POM121 becomes proteolytically degraded in a caspase-dependent manner. The POM121 antibodies and antibodies specific for other proteins of the nuclear envelope were used in a comparative study of nuclear apoptosis in staurosporine-treated buffalo rat liver cells. Nuclei from these cells were classified in three different stages of apoptotic progression: stage I, moderately condensed chromatin surrounded by a smooth nuclear periphery; stage II, compact patches of condensed chromatin collapsing against a smooth nuclear periphery; stage III, round compact chromatin bodies surrounded by grape-shaped nuclear periphery. We have performed double labeling immunofluorescence microscopy of individual apoptotic cells and quantitative immunoblotting analysis of total proteins from apoptotic cell cultures. The results showed that degradation of nuclear envelope marker proteins occurred in a specific order. POM121 degradation occurred surprisingly early and was initiated before nucleosomal DNA degradation could be detected using TUNEL assay and completed before clustering of the nuclear pores. POM121 was eliminated significantly more rapid compared with NUP153 (a peripheral protein located in the nucleoplasmic basket of the nuclear pore complex) and lamin B (a component of the nuclear lamina). Disappearance of NUP153 and lamin B was coincident with onset of DNA fragmentation and clustering of nuclear pores. By contrast, the peripheral NPC protein p62 was degraded much later. The results suggest that degradation of POM121 may be an important early step in propagation of nuclear apoptosis.

Place, publisher, year, edition, pages
2001. Vol. 114, no 20, 3643-3653 p.
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:sh:diva-15844ISI: 000171889300008PubMedID: 11707516Scopus ID: 2-s2.0-0034757949OAI: oai:DiVA.org:sh-15844DiVA: diva2:509188
Available from: 2012-03-12 Created: 2012-03-09 Last updated: 2016-12-22Bibliographically approved
In thesis
1. Distribution and dynamics of a nuclear pore membrane protein
Open this publication in new window or tab >>Distribution and dynamics of a nuclear pore membrane protein
2002 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Stockholm: Stockholms universitet, 2002. 42 p.
Keyword
Proteiner
National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-31517 (URN)91-7265-391-4 (ISBN)
Public defence
2002-02-08, Magnélisalen, Kemiska Övningslaboratoriet, Svante Arrheniusväg 12, Stockholm, 10:00
Opponent
Supervisors
Available from: 2016-12-22 Created: 2016-12-22 Last updated: 2016-12-22Bibliographically approved
2. Targeting of a nascent integral membrane protein to the nuclear pores and its degradation during apoptosis
Open this publication in new window or tab >>Targeting of a nascent integral membrane protein to the nuclear pores and its degradation during apoptosis
2002 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Stockholm: Stockholms universitet, 2002. 46 p.
National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-31521 (URN)91-7265-559-3 (ISBN)
Public defence
2002-12-13, Magnélisalen, Kemiska Övningslaboratoriet, Svante Arrenihusväg 12, Stockholm, 10:00
Opponent
Supervisors
Available from: 2016-12-22 Created: 2016-12-22 Last updated: 2016-12-22Bibliographically approved

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CiteExportLink to record
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