D1' centers are less efficient than normal photosystem II centers
2001 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 505, no 1, 113-117 p.Article in journal (Refereed) Published
One prominent difference between the photosystem II (PSII) reaction center protein D1 ' in Synechocystis 6803 and normal D1 is the replacement of Phe-186 in D1 with leucine in D1 '. Mutants of Synechocystis 6803 producing only D1 ', or containing engineered D1 proteins with Phe-186 substitutions, were analyzed by 77 K fluorescence emission spectra, chlorophyll a fluorescence induction yield and decay kinetics, and flash-induced oxygen evolution. Compared to D1-containing PSII centers, D1 ' centers exhibited a 50% reduction in variable chlorophyll a fluorescence yield, while the flash-induced O-2 evolution pattern was unaffected. In the F186 mutants, both the P680(+)/Q(A)(-) recombination and O-2 oscillation pattern were noticeably perturbed.
Place, publisher, year, edition, pages
2001. Vol. 505, no 1, 113-117 p.
Biochemistry and Molecular Biology Cell Biology Biophysics
IdentifiersURN: urn:nbn:se:sh:diva-15845DOI: 10.1016/S0014-5793(01)02794-6ISI: 000171084200022PubMedID: 11557052ScopusID: 2-s2.0-0035822896OAI: oai:DiVA.org:sh-15845DiVA: diva2:509051