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Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells
European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD), National Institutes of Health (NIH) - USA.
Södertörn University, Avdelning Naturvetenskap.
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2001 (English)In: Journal of Cell Biology, ISSN 0021-9525, E-ISSN 1540-8140, Vol. 154, no 1, 71-84 p.Article in journal (Refereed) Published
Abstract [en]

The nuclear pore complex (NPC) and its relationship to the nuclear envelope (NE) was characterized in living cells using POM121-green fluorescent protein (GFP) and GFP-Nup153, and GFP-lamin B1. No independent movement of single pore complexes was found within the plane of the NE in interphase. Only large arrays of NPCs moved slowly and synchronously during global changes in nuclear shape, strongly suggesting mechanical connections which form an NPC network. The nuclear lamina exhibited identical movements. NPC turnover measured by fluorescence recovery after photobleaching of POM121 was less than once per cell cycle. Nup153 association with NPCs was dynamic and turnover of this nucleoporin was three orders of magnitude faster. Overexpression of both nucleoporins induced the formation of annulate lamellae (AL) in the endoplasmic reticulum (ER). Turnover of AL pore complexes was much higher than in the NE (once every 2.5 min). During mitosis, POM121 and Nup153 were completely dispersed and mobile in the ER (POM121) or cytosol (Nup153) in metaphase, and rapidly redistributed to an immobilized pool around chromatin in late anaphase. Assembly and immobilization of both nucleoporins occurred before detectable recruitment of lamin B1, which is thus unlikely to mediate initiation of NPC assembly at the end of mitosis.

Place, publisher, year, edition, pages
2001. Vol. 154, no 1, 71-84 p.
National Category
Cell Biology
URN: urn:nbn:se:sh:diva-15852DOI: 10.1083/jcb.200101089ISI: 000169845900013PubMedID: 11448991ScopusID: 2-s2.0-0035833254OAI: diva2:509030
Available from: 2012-03-12 Created: 2012-03-09 Last updated: 2016-12-22Bibliographically approved
In thesis
1. Distribution and dynamics of a nuclear pore membrane protein
Open this publication in new window or tab >>Distribution and dynamics of a nuclear pore membrane protein
2002 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Stockholm: Stockholms universitet, 2002. 42 p.
National Category
Biological Sciences
urn:nbn:se:sh:diva-31517 (URN)91-7265-391-4 (ISBN)
Public defence
2002-02-08, Magnélisalen, Kemiska Övningslaboratoriet, Svante Arrheniusväg 12, Stockholm, 10:00
Available from: 2016-12-22 Created: 2016-12-22 Last updated: 2016-12-22Bibliographically approved

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Imreh, GabrielaHallberg, Einar
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