Loss of p53 compensates for alpha(v)-integrin function in retinal neovascularization
2002 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 277, no 16, 13371-13374 p.Article in journal (Refereed) Published
alpha(v)-Integrin antagonists block neovascularization in various species, whereas 20% of alpha(v)-integrin null mice are born with many normal looking blood vessels. Given that blockade of alpha(v)-integrins during angiogenesis induces p53 activity, we utilized p53 null mice to elucidate whether loss of p53 can compensate for av-integrin function in neovascularization of the retina. Murine retinal vascularization was inhibited by systemic administration of an alpha(v)-integrin antagonist. In contrast, mice lacking p53 were refractory to this treatment, indicating that neovascularization in normal mice depends on alpha(v)-integrin-mediated suppression of p53. Blockade of alpha(v)-integrins during neovascularization resulted in an induction of p21(CIP1) in wild type and, surprisingly, in p53 null retinas, indicating that alpha(v)-integrin ligation regulates p21(CIP1) levels in a p53-independent manner. In conclusion, we demonstrate for the first time an in vivo intracellular mechanism for compensation of integrin function and that p53 and alpha(v)-integrins act in concert during retinal neovascularization.
Place, publisher, year, edition, pages
2002. Vol. 277, no 16, 13371-13374 p.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:sh:diva-15806DOI: 10.1074/jbc.C200044200ISI: 000175096000004PubMedID: 11856728OAI: oai:DiVA.org:sh-15806DiVA: diva2:508482