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Proteome map of the chloroplast lumen of Arabidopsis thaliana
Södertörn University, Avdelning Naturvetenskap. Karolinska Institute.
Södertörn University, Avdelning Naturvetenskap. Stockholm University.
Institute for Genomic Research, Rockville, USA.
Stockholm University.
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2002 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 277, no 10, 8354-8365 p.Article in journal (Refereed) Published
Abstract [en]

The thylakoid membrane of the chloroplast is the center of oxygenic photosynthesis. To better understand the function of the luminal compartment within the thylakoid network, we have carried out a systematic characterization of the luminal thylakoid proteins from the model organism Arabidopsis thaliana. Our data show that the thylakoid lumen has its own specific proteome, of which 36 proteins were identified. Besides a large group of peptidyl-prolyl cis-trans isomerases and pro. teases, a family of novel PsbP domain proteins was found. An analysis of the luminal signal peptides showed that 19 of 36 luminal precursors were marked by a twin-arginine motif for import via the Tat pathway. To compare the model organism Arabidopsis with another typical higher plant, we investigated the proteome from the thylakoid lumen of spinach and found that the luminal proteins from both plants corresponded well. As a complement to our experimental investigation, we made a theoretical prediction of the luminal proteins from the whole Arabidopsis genome and estimated that the thylakoid lumen of the chloroplast contains similar to80 proteins.

Place, publisher, year, edition, pages
2002. Vol. 277, no 10, 8354-8365 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:sh:diva-15812DOI: 10.1074/jbc.M108575200ISI: 000174268000094PubMedID: 11719511ScopusID: 2-s2.0-0037040944OAI: oai:DiVA.org:sh-15812DiVA: diva2:508477
Available from: 2012-03-08 Created: 2012-03-07 Last updated: 2016-12-20Bibliographically approved
In thesis
1. Overview and characterisation of the thylakoid lumen of higher plants
Open this publication in new window or tab >>Overview and characterisation of the thylakoid lumen of higher plants
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In higher plants, chloroplasts are the site for the photosynthetic reactions, converting solar energy to chemical energy. Within the chloroplast the thylakoid membrane network encloses the soluble lumen compartment. Until recently the knowledge of the lumen composition and function was limited, but a more profound understanding of the thylakoid lumen content is gradually emerging. The discovery that the thylakoid lumen contains numerous enzymes, besides the already known proteins directly involved or associated with the photosynthetic reactions, have changed the view on this compartment.

The first part of the thesis the lumen proteome maps of Arabidopsis and spinach were resolved. These two proteome maps showed good correlation and the same protein groups were represented in the two proteomes. Thirty eight proteins were identified and in combination with an in silico prediction for the proteome it was estimated that at least 80 different proteins are lumen located.

The second part was to further investigate the functions of two lumen localized proteins, the cyclophilin AtCYP20-2 and peroxiredoxin Q (PrxQ). AtCYP20-2 is suggested to be responsible for the major peptidyl prolyl cis/trans isomerases (PPIase) activity in the lumen. In AtCYP20-2 knockout mutants the total PPIase activity is not altered, instead AtFKBP13 may be oxidatively activated to compensate for the loss of AtCYP20-2. With respect to the PrxQ protein we were able to show that it is a lumenal protein that seems to exist as a soluble pool in the lumen as no interaction with the thylakoid membrane could be detected.

In the last part of this thesis the dynamics of the thylakoid membrane and lumen proteome were studied during oxidative stress. Important changes to the proteome were observed in response to the stress treatment, including abundance changes of proteins related to photosynthesis, as well as proteins not previously identified in relation to stress.

Place, publisher, year, edition, pages
Stockholm: Stockholm University, 2006. 51 p.
Keyword
Arablidopsis, thylakoid lumen, AtCYP20-2, Peroxiredoxin Q, oxidative stress
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:sh:diva-31471 (URN)91-7155-299-5 (ISBN)
Public defence
2006-11-24, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 12 A, 10:00
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Available from: 2016-12-20 Created: 2016-12-20 Last updated: 2016-12-20Bibliographically approved

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