Dicer is required for chromosome segregation and gene silencing in fission yeast cells
2002 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 99, no 26, 16648-16653 p.Article in journal (Refereed) Published
RNA interference is a form of gene silencing in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs. Here we report a role for Dicer in chromosome segregation of fission yeast. Deletion of the Dicer (dcr1(+)) gene caused slow growth, sensitivity to thiabendazole, lagging chromosomes during anaphase, and abrogated silencing of centromeric repeats. As Dicer in other species, Dcr1p degraded double-stranded RNA into approximate to23 nucleotide fragments in vitro, and dcr1Delta cells were partially rescued by expression of human Dicer, indicating evolutionarily conserved functions. Expression profiling demonstrated that dcr1(+) was required for silencing of two genes containing a conserved motif.
Place, publisher, year, edition, pages
2002. Vol. 99, no 26, 16648-16653 p.
IdentifiersURN: urn:nbn:se:sh:diva-15719DOI: 10.1073/pnas.212633199ISI: 000180101600028PubMedID: 12482946ScopusID: 2-s2.0-0037168521OAI: oai:DiVA.org:sh-15719DiVA: diva2:508475