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Recruitment of Gen5-containing complexes during c-Myc-dependent gene activation - Structure and function aspects
Södertörn University, Avdelning Naturvetenskap. Karolinska Intstitutet.
Södertörn University, Avdelning Naturvetenskap. Karolinska Institutet.
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2002 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 277, no 26, 23399-23406 p.Article in journal (Refereed) Published
Abstract [en]

The N-terminal domain of c-Myc plays a key role in cellular transformation and is involved in both activation and repression of target genes as well as in modulated proteolysis of c-Myc via the proteasome. Given this functional complexity, it has been difficult to clarify the structures within the N terminus that contribute to these different processes as well as the mechanisms by which they function. We have used a simplified yeast model system to identify the primary determinants within the N terminus for W chromatin remodeling of a promoter, (ii) gene activation from a chromatin template in vivo, and (iii) interaction with highly purified Gcn5 complexes as well as other chromatin-remodeling complexes in vitro. The results identify two regions that contain autonomous chromatin opening and gene activation activity, but both regions are required for efficient interaction with chromatin-remodeling complexes in vitro. The conserved Myc boxes do not play a direct role in gene activation, and Myc box II is not generally required for in vitro interactions with remodeling complexes. The yeast SAGA complex, which is orthologous to the human GCN5-TRRAP complex that interacts with Myc in human cells, plays a role in Myc-mediated chromatin opening at the promoter but may also be involved in later steps of gene activation.

Place, publisher, year, edition, pages
2002. Vol. 277, no 26, 23399-23406 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:sh:diva-15799DOI: 10.1074/jbc.M201704200ISI: 000176475700040PubMedID: 11973336Scopus ID: 2-s2.0-0037189508OAI: oai:DiVA.org:sh-15799DiVA: diva2:508380
Available from: 2012-03-08 Created: 2012-03-07 Last updated: 2017-06-29Bibliographically approved

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Flinn, Elizabeth MHermann, StefanWright, Anthony P H
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CiteExportLink to record
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Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
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