Architectural principles for the structure and function of the glucocorticoid receptor tau 1 core activation domain
2000 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 275, no 20, 15014-15018 p.Article in journal (Refereed) Published
A 58-amino acid region mediates the core transactivation activity of the glucocorticoid receptor tau 1 activation domain. This tau 1 core domain is unstructured in aqueous buffers, but in the presence of trifluoroethanol three Alpha-helical segments are induced. Two of these putative structural modules have been tested in different combinations with regard to transactivation potential in vivo and binding capacity to the coactivators in vitro, The results show that whereas single modules are not transcriptionally active, any combination of two or three modules is sufficient, with trimodular constructs having the highest activity. However, proteins containing one, two, or three segments bind Ada2 and cAMP-response element-binding protein with similar affinity. A single segment is thus able to bind a target factor but cannot transactivate target genes significantly. The results are consistent with models in which activation domains are comprised of short activation modules that allow multiple interactions with coactivators. Our results also suggest that an increased number of modules may not result in correspondingly higher affinity but instead that the concentration of binding sites is increased, which gives rise to a higher association rate. This is consistent with a model where the association rate for activator-target factor interactions rather than the equilibrium constant is the most relevant measure of activator potency.
Place, publisher, year, edition, pages
2000. Vol. 275, no 20, 15014-15018 p.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:sh:diva-15751DOI: 10.1074/jbc.M001007200ISI: 000087128300035PubMedID: 10747977ScopusID: 2-s2.0-0040784176OAI: oai:DiVA.org:sh-15751DiVA: diva2:508114