sh.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus influenzae
Södertörn University, Avdelning Naturvetenskap. Karolinska Institutet.
John Radcliffe Hospital, Oxford, UK.
John Radcliffe Hospital, Oxford, UK.
Södertörn University, Avdelning Naturvetenskap. Karolinska Institutet.
2003 (English)In: European Journal of Biochemistry, ISSN 0014-2956, E-ISSN 1432-1033, Vol. 270, no 14, 2979-2991 p.Article in journal (Refereed) Published
Abstract [en]

Structural analysis of the lipopolysaccharide (LPS) from nontypeable Haemophilus influenzae strain 981 has been achieved using NMR spectroscopy and ESI-MS on O-deacylated LPS and core oligosaccharide (OS) material as well as by ESI-MSn on permethylated dephosphorylated OS. A heterogeneous glycoform population was identified, resulting from the variable length of the OS branches attached to the glucose residue in the common structural element of H. influenzae LPS, L-alpha-d-Hepp -(1-->2)-[P Etn-->6]-L-alpha-d-Hepp -(1-->3)-[beta-d-Glcxp-(1-->4)]-L-alpha-d-Hepp -(1-->5)-[PP Etn-->4]-alpha-Kdop -(2-->6)-Lipid A. Notably, the O-6 position of the beta-d-Glcp residue was either substituted by P Cho or the disaccharide branch beta-d-Galp-(1-->4)-d-alpha-d-Hepp, while the O-4 position was substituted by the globotetraose unit, beta-d-Galp NAc-(1-->3)-alpha-d-Galp -(1-->4)-beta-d-Galp -(1-->4)-beta-d-Glcp, or sequentially truncated versions thereof. This is the first time a branching sugar residue has been reported in the outer-core region of H. influenzae LPS. Additionally, a P Etn group was identified at O-3 of the distal heptose residue in the inner-core.

Place, publisher, year, edition, pages
2003. Vol. 270, no 14, 2979-2991 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:sh:diva-15541DOI: 10.1046/j.1432-1033.2003.03675.xISI: 000183959900008PubMedID: 12846831Scopus ID: 2-s2.0-19244383477OAI: oai:DiVA.org:sh-15541DiVA: diva2:504731
Available from: 2012-02-21 Created: 2012-02-21 Last updated: 2017-02-15Bibliographically approved
In thesis
1. The structural diversity of lipopolysaccharides expressed by genetically defined clinical isolates of nontypeable Haemophilus influenzae
Open this publication in new window or tab >>The structural diversity of lipopolysaccharides expressed by genetically defined clinical isolates of nontypeable Haemophilus influenzae
2003 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Nontypeable Haemophilus influenzae (NTHi) is an important cause of otitis media and respiratory tract infections. Its lipopolysaccharide (LPS) molecule, an outer membrane component, is a major virulence factor of NTHi. The LPS molecule may also be an efficient target for antibodies which might be protective against NTHi disease. The present thesis describes the structural analyses of the LPS from a number of NTHi clinical isolates. These isolates have been selected to be representative of the genetic diversity in the natural population of NTHi. The studies revealed extended inter- and intrastrain variability in LPS expression but also the presence of a conserved structural element. Several novel structural features were found, including epitopes not previously observed in Haemophilus influenzae LPS. The studies were performed using nuclear magnetic resonance spectroscopy, electrospray ionization mass spectrometry and different chemical degradations of the LPS as the principal methods.

Place, publisher, year, edition, pages
Stockholm: Karolinska Instiutet, 2003. 52 p.
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:sh:diva-32070 (URN)91-7349-584-0 (ISBN)
Public defence
2003-08-29, Hörsalen, plan 4, NOVUM, Hälsovägen 7, Huddinge, 10:00 (English)
Opponent
Supervisors
Available from: 2017-02-15 Created: 2017-02-15 Last updated: 2017-02-15Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Månsson, MartinSchweda, Elke K H
By organisation
Avdelning Naturvetenskap
In the same journal
European Journal of Biochemistry
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 70 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf