Overexpression of Bcl-2 in T cells affects insulitis in the nonobese diabetic mouse
2003 (English)In: Scandinavian Journal of Immunology, ISSN 0300-9475, E-ISSN 1365-3083, Vol. 57, no 4, 342-349 p.Article in journal (Refereed) Published
The nonobese diabetic (NOD) mouse is a useful model for human autoimmune diabetes. The gene for the anti-apoptotic protein Bcl-2 has previously been suggested as a probable susceptibility candidate for the NOD mouse disease. In this study, we investigated how overexpression of Bcl-2 in lymphocytes might affect insulitis in NOD mice. A bcl-2 transgene expressed constitutively under the SV40-promoter and the 5'Igh enhancer, Emu, was bred onto NOD background. Two bcl-2 transgenic NOD strains were produced and analysed, one with overexpression of Bcl-2 on only B cells and the other with overexpression of Bcl-2 on both B and T cells. Subsequent to verification of expression pattern and functionality of the transgene, insulitis intensity was investigated in different backcross generations of the two transgenic strains. Overexpression of Bcl-2 on both B and T cells leads to a statistically significant protection of the mice from insulitis compared with normal littermates. Overexpression of Bcl-2 on only B cells, on the other hand, does not have any statistically significant effect on insulitis. Possible mechanisms for the effect of Bcl-2 on insulitis in NOD mice are discussed.
Place, publisher, year, edition, pages
2003. Vol. 57, no 4, 342-349 p.
IdentifiersURN: urn:nbn:se:sh:diva-15548DOI: 10.1046/j.1365-3083.2003.01244.xISI: 000181862300006PubMedID: 12662297ScopusID: 2-s2.0-0037384047OAI: oai:DiVA.org:sh-15548DiVA: diva2:504718