Change search
ReferencesLink to record
Permanent link

Direct link
Reduction of cholesterol and glycoalkaloid levels in transgenic potato plants by overexpression of a type 1 sterol methyltransferase cDNA
Södertörn University, Avdelning Naturvetenskap.
2003 (English)In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 131, no 4, 1792-1799 p.Article in journal (Refereed) Published
Abstract [en]

Transgenic potato (Solanum tuberosum cv Desiree) plants overexpressing a soybean (Glycine max) type 1 sterol methyltransferase (GmSMT1) cDNA were generated and used to study sterol biosynthesis in relation to the production of toxic glycoalkaloids. Transgenic plants displayed an increased total sterol level in both leaves and tubers, mainly due to increased levels of the 24-ethyl sterols isofucosterol and sitosterol. The higher total sterol level was due to increases in both free and esterified sterols. However, the level of free cholesterol, a nonalkylated sterol, was decreased. Associated with this was a decreased glycoalkaloid level in leaves and tubers, down to 41% and 63% of wild-type levels, respectively. The results show that glycoalkaloid biosynthesis can be down-regulated in transgenic potato plants by reducing the content of free nonalkylated sterols, and they support the view of cholesterol as a precursor in glycoalkaloid biosynthesis.

Place, publisher, year, edition, pages
2003. Vol. 131, no 4, 1792-1799 p.
National Category
URN: urn:nbn:se:sh:diva-15553DOI: 10.1104/pp.102.018788ISI: 000185076400029PubMedID: 166935ScopusID: 2-s2.0-0344942508OAI: diva2:504716
Available from: 2012-02-21 Created: 2012-02-21 Last updated: 2014-04-16Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Jonsson, Lisbeth
By organisation
Avdelning Naturvetenskap
In the same journal
Plant Physiology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 117 hits
ReferencesLink to record
Permanent link

Direct link