sh.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Vilse, a conserved Rac/Cdc42 GAP mediating Robo repulsion in tracheal cells and axons
Södertörn University, School of Chemistry, Biology, Geography and Environmental Science.
Show others and affiliations
2004 (English)In: Genes & Development, ISSN 0890-9369, E-ISSN 1549-5477, Vol. 18, no 17, 2161-2171 p.Article in journal (Refereed) Published
Abstract [en]

Slit proteins steer the migration of many cell types through their binding to Robo receptors, but how Robo controls cell motility is not clear. We describe the functional analysis of vilse, a Drosophila gene required for Robo repulsion in epithelial cells and axons. Vilse defines a conserved family of RhoGAPs (Rho GTPase-activating proteins), with representatives in flies and vertebrates. The phenotypes of vilse mutants resemble the tracheal and axonal phenotypes of Slit and Robo mutants at the CNS midline. Dosage-sensitive genetic interactions between vilse, slit, and robo mutants suggest that vilse is a component of robo signaling. Moreover, overexpression of Vilse in the trachea of robo mutants ameliorates the phenotypes of robo, indicating that Vilse acts downstream of Robo to mediate midline repulsion. Vilse and its human homolog bind directly to the intracellular domains of the corresponding Robo receptors and promote the hydrolysis of RacGTP and, less efficiently, of Cdc42GTP. These results together with genetic interaction experiments with robo, vilse, and rac mutants suggest a mechanism whereby Robo repulsion is mediated by the localized inactivation of Rac through Vilse.

Place, publisher, year, edition, pages
2004. Vol. 18, no 17, 2161-2171 p.
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:sh:diva-15468DOI: 10.1101/gad.310204ISI: 000223675200012PubMedID: 15342493OAI: oai:DiVA.org:sh-15468DiVA: diva2:504683
Available from: 2012-02-21 Created: 2012-02-20 Last updated: 2017-07-19Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedPMC Full text

Search in DiVA

By author/editor
Gallio, Marco
By organisation
School of Chemistry, Biology, Geography and Environmental Science
In the same journal
Genes & Development
Cell Biology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 55 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf