sh.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
Degradation of 4-chlorophenol at low temperature and during extreme temperature fluctuations by Arthrobacter chlorophenolicus A6
Södertörn University, School of Chemistry, Biology, Geography and Environmental Science. Karolinska Institutet.
Södertörn University, School of Chemistry, Biology, Geography and Environmental Science. SLU.
2004 (English)In: Microbial Ecology, ISSN 0095-3628, E-ISSN 1432-184X, Vol. 48, no 2, 246-253 p.Article in journal (Refereed) Published
Abstract [en]

Low average temperatures and temperature fluctuations in temperate soils challenge the efficacy of microbial strains used for clean up of pollutants. In this study, we investigated the cold tolerance of Arthrobacter chlorophenolicus A6, a microorganism previously shown to degrade high concentrations of 4-chlorophenol at 28degreesC. Luciferase activity from a luc-tagged derivative of the strain (A6L) was used to monitor the metabolic status of the population during 4-chlorophenol degradation. The A6L strain could degrade 200-300 mug mL(-1) 4-chlorophenol in pure cultures incubated at 5degreesC, although rates of degradation, growth and the metabolic status of the cells were lower at 5degreesC compared to 28degreesC. When subjected to temperature fluctuations between 5 and 28degreesC, A6L continued to degrade 4-chlorophenol and remained active. In soil microcosm experiments, the degradation rates were significantly faster the first week at 28degreesC, compared to 5degreesC. However, this difference was no longer seen after 7 days, and equally low 4-chlorophenol concentrations were reached after 17 days at both temperatures. During 4-chlorophenol degradation in soil, CFU and luciferase activity values remained constant at both 5 and 28degreesC. However, once most of the 4-chlorophenol was degraded, both values decreased by 1-1.5 logarithmic values at 28degreesC, whereas they remained constant at 5degreesC, indicating a high survival of the cells at low temperatures. Because of the ability of A. chlorophenolicus A6 to degrade high concentrations of 4-chlorophenol at 5degreesC, together with its tolerance to temperature fluctuations and stress conditions found in soil, this strain is a promising candidate for bioaugmentation of chlorophenol-contaminated soil in temperate climates.

Place, publisher, year, edition, pages
2004. Vol. 48, no 2, 246-253 p.
National Category
Environmental Sciences Microbiology
Identifiers
URN: urn:nbn:se:sh:diva-15470DOI: 10.1007/s00248-003-2026-3ISI: 000224863800011PubMedID: 15546043ScopusID: 2-s2.0-16644389032OAI: oai:DiVA.org:sh-15470DiVA: diva2:504679
Available from: 2012-02-21 Created: 2012-02-20 Last updated: 2014-04-16Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Backman, AgnetaJansson, Janet K
By organisation
School of Chemistry, Biology, Geography and Environmental Science
In the same journal
Microbial Ecology
Environmental SciencesMicrobiology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 57 hits
ReferencesLink to record
Permanent link

Direct link