Degradation of GFP-labelled POM121, a non-invasive sensor of nuclear apoptosis, precedes clustering of nuclear pores and externalisation of phosphatidylserine
2004 (English)In: Apoptosis (London), ISSN 1360-8185, E-ISSN 1573-675X, Vol. 9, no 3, 363-368 p.Article in journal (Refereed) Published
The nuclear pore membrane protein POM121 is specifically degraded during apoptosis by a caspase-3-dependent process enabling early detection of apoptosis in living cells expressing POM121-GFP. Here we further investigated temporal aspects of apoptotic degradation of POM121-GFP. We demonstrate that decreased POM121-GFP fluorescence precedes annexin V-labelling of apoptotic cells. This indicates that degradation of the nuclear pore complex starts prior to redistribution of plasma membrane phosphatidylserine, which serves as a signal for phagocytotic elimination of apoptotic cells. Furthermore, a caspase-resistant GFP-labelled mutant of POM121 resisted degradation even in late apoptosis and was detected in clustered nuclear pores. Thus, it can be concluded that loss of POM121-GFP is a specific sensor of the activation of caspase-3-dependent proteolysis at the nuclear pores.
Place, publisher, year, edition, pages
2004. Vol. 9, no 3, 363-368 p.
Biochemistry and Molecular Biology Cell Biology
IdentifiersURN: urn:nbn:se:sh:diva-15480DOI: 10.1023/B:APPT.0000025813.75258.b5ISI: 000221102700012PubMedID: 15258468ScopusID: 2-s2.0-3242805164OAI: oai:DiVA.org:sh-15480DiVA: diva2:504654