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Degradation of GFP-labelled POM121, a non-invasive sensor of nuclear apoptosis, precedes clustering of nuclear pores and externalisation of phosphatidylserine
Södertörn University, School of Chemistry, Biology, Geography and Environmental Science.
Södertörn University, School of Chemistry, Biology, Geography and Environmental Science.
2004 (English)In: Apoptosis (London), ISSN 1360-8185, E-ISSN 1573-675X, Vol. 9, no 3, 363-368 p.Article in journal (Refereed) Published
Abstract [en]

The nuclear pore membrane protein POM121 is specifically degraded during apoptosis by a caspase-3-dependent process enabling early detection of apoptosis in living cells expressing POM121-GFP. Here we further investigated temporal aspects of apoptotic degradation of POM121-GFP. We demonstrate that decreased POM121-GFP fluorescence precedes annexin V-labelling of apoptotic cells. This indicates that degradation of the nuclear pore complex starts prior to redistribution of plasma membrane phosphatidylserine, which serves as a signal for phagocytotic elimination of apoptotic cells. Furthermore, a caspase-resistant GFP-labelled mutant of POM121 resisted degradation even in late apoptosis and was detected in clustered nuclear pores. Thus, it can be concluded that loss of POM121-GFP is a specific sensor of the activation of caspase-3-dependent proteolysis at the nuclear pores.

Place, publisher, year, edition, pages
2004. Vol. 9, no 3, 363-368 p.
National Category
Biochemistry and Molecular Biology Cell Biology
Identifiers
URN: urn:nbn:se:sh:diva-15480DOI: 10.1023/B:APPT.0000025813.75258.b5ISI: 000221102700012PubMedID: 15258468ScopusID: 2-s2.0-3242805164OAI: oai:DiVA.org:sh-15480DiVA: diva2:504654
Available from: 2012-02-21 Created: 2012-02-20 Last updated: 2014-04-16Bibliographically approved

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Kihlmark, MadeleineHallberg, Einar
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