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A novel type of silencing factor, Clr2, is necessary for transcriptional silencing at various chromosomal locations in the fission yeast Schizosaccharomyces pombe
Södertörn University, School of Chemistry, Biology, Geography and Environmental Science. Uppsala University / University of Copenhagen, Denmark .
Södertörn University, School of Chemistry, Biology, Geography and Environmental Science.
2004 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 32, no 15, 4421-4428 p.Article in journal (Refereed) Published
Abstract [en]

The mating-type region of the fission yeast Schizosaccharomyces pombe comprises three loci: mat1, mat2-P and mat3-M. mat1 is expressed and determines the mating type of the cell. mat2-P and mat3-M are two storage cassettes located in a 17 kb heterochromatic region with features identical to those of mammalian heterochromatin. Mutations in the swi6(+), clr1(+), clr2(+), clr3(+), clr4(+) and clr6(+) genes were obtained in screens for factors necessary for silencing the mat2-P-mat3-M region. swi6(+) encodes a chromodomain protein, clr3(+) and clr6(+) histone deacetylases, and clr4(+) a histone methyltransferase. Here, we describe the cloning and characterization of clr2(+). The clr2(+) gene encodes a 62 kDa protein with no obvious sequence homologs. Deletion of clr2(+) not only affects transcriptional repression in the mating-type region, but also centromeric silencing and silencing of a PolII-transcribed gene inserted in the rDNA repeats. Using chromatin immunoprecipitation, we show that Clr2 is necessary for histone hypoacetylation in the mating-type region, suggesting that Clr2 acts upstream of histone deacetylases to promote transcriptional silencing.

Place, publisher, year, edition, pages
2004. Vol. 32, no 15, 4421-4428 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:sh:diva-15504DOI: 10.1093/nar/gkh780ISI: 000223947100001PubMedID: 15317867Scopus ID: 2-s2.0-4243144074OAI: oai:DiVA.org:sh-15504DiVA: diva2:504334
Available from: 2012-02-20 Created: 2012-02-20 Last updated: 2017-07-19Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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