sh.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
Structural investigation of lipopolysaccharides from nontypeable Haemophilus influenzae: investigation of inner-core phosphoethanolamine addition in NTHi strain 981
Södertörn University, School of Life Sciences.
Södertörn University, School of Life Sciences.
Show others and affiliations
2005 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 340, no 11, 1900-1907 p.Article in journal (Refereed) Published
Abstract [en]

LPS of NTHi comprises a conserved tri-L-glycero-D-manno-heptosyl inner-core moiety (L-alpha-D-Hepp-(1 -> 2)-[PEtn -> 6]-L-alpha-D-Hepp-(1 -> 3)-[beta-D-Glcp-(1 -> 4)]-L-alpha-D-Hepp-(1 -> 5)-alpha-Kdop) in which addition of PEtn to the central heptose (HepII) in strain Rd is controlled by the gene lpt6. It was recently shown that NTHi strain 981 contains an additional PEtn linked to O-3 of the terminal heptose of the inner-core moiety (HepIII). In order to establish whether lpt6 is also involved in adding PEtn to HepIII, lpt6 in strain 981 was inactivated. The structure of the LPS of the resulting mutant strain 981lpt6 was investigated by MS and NMR techniques by which it was confirmed that the lpt6 gene product is responsible for addition of PEtn to O-6 of HepII in strain 981. However, it is not responsible for adding PEtn to O-3 of HepIII since the 981lpt6 mutant still had full substitution with PEtn at HepIII

Place, publisher, year, edition, pages
2005. Vol. 340, no 11, 1900-1907 p.
National Category
Biochemistry and Molecular Biology Organic Chemistry
Identifiers
URN: urn:nbn:se:sh:diva-14448DOI: 10.1016/j.carres.2005.06.007ISI: 000230952400019OAI: oai:DiVA.org:sh-14448DiVA: diva2:487409
Available from: 2012-01-31 Created: 2011-12-23 Last updated: 2012-01-31Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Månsson, MartinYildirim, Håkan HSchweda, Elke K H
By organisation
School of Life Sciences
In the same journal
Carbohydrate Research
Biochemistry and Molecular BiologyOrganic Chemistry

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 37 hits
ReferencesLink to record
Permanent link

Direct link