Change search
ReferencesLink to record
Permanent link

Direct link
Functional genomics of the cilium, a sensory organelle
Show others and affiliations
2005 (English)In: Current Biology, ISSN 0960-9822, E-ISSN 1879-0445, Vol. 15, no 10, 935-941 p.Article in journal (Refereed) Published
Abstract [en]

Cilia and flagella play important roles in many physiological processes, including cell and fluid movement, sensory perception, and development [1]. The biogenesis and maintenance of cilia depend on intraflagellar transport (IFT), a motility process that operates bidirectionally along the ciliary axoneme [1, 2]. Disruption in IFT and cilia function causes several human disorders, including polycystic kidneys, retinal dystrophy, neurosensory impairment, and Bardet-Bledl syndrome (BBS) [3-5]. To uncover new ciliary components, including IFT proteins, we compared C. elegans ciliated neuronal and nonciliated cells through serial analysis of gene expression (SAGE) and screened for genes potentially regulated by the cillogenic transcription factor, DAF-19 [6]. Using these complementary approaches, we identified numerous candidate ciliary genes and confirmed the ciliated-cell-specific expression of 14 novel genes. One of these, C27H5.7a, encodes a ciliary protein that undergoes IFT. As with other IFT proteins, its ciliary localization and transport is disrupted by mutations in IFT and bbs genes. Furthermore, we demonstrate that the ciliary structural defect of C. elegans dyf-13(mn396) mutants is caused by a mutation in C27H5.7a. Together, our findings help define a ciliary transcriptome and suggest that DYF-13, an evolutionarily conserved protein, is a novel core IFT component required for cilia function.

Place, publisher, year, edition, pages
2005. Vol. 15, no 10, 935-941 p.
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:sh:diva-14458DOI: 10.1016/j.cub.2005.04.059ISI: 000229446500023PubMedID: 15916950ScopusID: 2-s2.0-20144380575OAI: diva2:469392
Available from: 2011-12-23 Created: 2011-12-23 Last updated: 2014-04-16Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Swoboda, Peter
By organisation
School of Life Sciences
In the same journal
Current Biology
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 78 hits
ReferencesLink to record
Permanent link

Direct link