Electrophoretic and mass spectrometric strategies for profiling bacterial lipopolysaccharides
2005 (English)In: MOLECULAR BIOSYSTEMS, ISSN 1742-206X, Vol. 1, no 1, 46-52 p.Article in journal (Refereed) Published
Capillary electrophoresis (CE) is a high-resolution separation technique that has been widely used for trace analysis in biological samples. On-line capillary electrophoresis-electro spray mass spectrometry (CE-MS) was developed for the analysis of lipopolysaccharide (LPS) glycoforms from the gram-negative bacteria, Haemophilus influenzae. In this paper, we report on the application of CE-MS to characterize structural differences in O-deacylated LPS samples from H. influenzae strains Rd 11.7 and 375.1. The resolution capability of on-line CE-MS was first demonstrated by analysis of a complex LPS mixture from H. influenzae strain Rd 11.7. This strain contains a mixture of isomeric glycoforms differing in the number and positions of hexose moieties. Sialic acid containing glycoforms were also determined. Structural features of LPS from a lic1 mutant of H. influenzae strain 375 (375.1) were studied using on-line CE-MS/MS. With the separation provided by CE, two isomeric glycoforms differing in the location of phosphoethanolamine substituents were characterized by tandem mass spectrometry.
Place, publisher, year, edition, pages
2005. Vol. 1, no 1, 46-52 p.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:sh:diva-14464DOI: 10.1039/b501686jISI: 000233977800006OAI: oai:DiVA.org:sh-14464DiVA: diva2:469387