Increased conjugation frequencies in clinical Enterococcus faecium strains harbouring the enterococcal surface protein gene esp
2006 (English)In: Clinical Microbiology and Infection, ISSN 1198-743X, E-ISSN 1469-0691, Vol. 12, no 6, 588-591 p.Article in journal (Refereed) Published
This study compared the in-vitro ability of Enterococcus faecium isolates of different origin to acquire vanA by conjugation in relation to the occurrence of the esp gene. In total, 29 clinical isolates (15/29 esp+), 30 normal intestinal microflora isolates (2/30 esp+) and one probiotic strain (esp-) were studied with a filter-mating assay. Conjugation events were confirmed by PCR and pulsed-field gel electrophoresis. Among the infection-derived isolates, the esp+ isolates had higher conjugation frequencies compared with esp- isolates (p < 0.001), with a median value of 6.4 x 10(-6) transconjugants/donor. The probiotic strain was shown to acquire vanA vancomycin resistance in in-vitro filter mating experiments.
Place, publisher, year, edition, pages
2006. Vol. 12, no 6, 588-591 p.
IdentifiersURN: urn:nbn:se:sh:diva-14298DOI: 10.1111/j.1469-0691.2006.01436.xISI: 000237515600017PubMedID: 16700712ScopusID: 2-s2.0-33646702110OAI: oai:DiVA.org:sh-14298DiVA: diva2:468161