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The hedgehog-related gene wrt-5 is essential for hypodermal development in Caenorhabditis elegans
Södertörn University, School of Life Sciences. Karolinska Institute.
2006 (English)In: Developmental Biology, ISSN 0012-1606, E-ISSN 1095-564X, Vol. 290, no 2, 323-336 p.Article in journal (Refereed) Published
Abstract [en]

The Caenorhabditis elegans genome encodes a series of hedgehog-related genes, which are thought to have evolved and diverged from an ancestral Hh gene. They are classified into several families based on their N-terminal domains. Here, we analyze the expression and function of a member of the warthog gene family, wrt-5, that lacks the Hint/Hog domain. wrt-5 is expressed in seam cells, the pharynx, pharyngeal-intestinal valve cells, neurons, neuronal support cells, the excretory cell, and the reproductive system. WRT-5 protein is secreted into the extracelluar space during embryogenesis. Furthermore, during larval development, WRT-5 protein is secreted into the pharyngeal lumen and the pharyngeal expression changes in a cyclical manner in phase with the molting cycle. Deletion mutations in wrt-5 cause embryonic lethality, which are temperature sensitive and more severe at 15 degrees C than at 25 degrees C. Animals that hatch exhibit variable abnormal morphology, for example, bagging worms, blistering, molting defects, or Roller phenotypes. We examined hypodermal cell junctions using the AJM-1: :GFP marker in the wrt-5 mutant background and observed cell boundary abnormalities in the arrested embryos. AJM-1: :GFP protein is also misplaced in pharyngeal muscle cells in the absence of WRT-5. In conclusion, we show that wrt-5 is an essential gene that - despite its lack of a Hint domain - has multiple functions in C. elegans and is implicated in cell shape integrity.

Place, publisher, year, edition, pages
2006. Vol. 290, no 2, 323-336 p.
National Category
Developmental Biology
Identifiers
URN: urn:nbn:se:sh:diva-14307DOI: 10.1016/j.ydbio.2005.11.028ISI: 000235382500008PubMedID: 2-s2.0-31544472858ScopusID: 2-s2.0-31544472858OAI: oai:DiVA.org:sh-14307DiVA: diva2:468123
Available from: 2011-12-20 Created: 2011-12-20 Last updated: 2014-03-17Bibliographically approved

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Bürglin, Thomas R
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