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The arginine methyltransferase Rmt2 is enriched in the nucleus and co-purifies with the nuclear porins Nup49, Nup57 and Nup100
Södertörn University, School of Life Sciences.
Södertörn University, School of Life Sciences.
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2007 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 313, no 9, 1778-1789 p.Article in journal (Refereed) Published
Abstract [en]

Arginine methylation is a post-translational modification of proteins implicated in RNA processing, protein compartmentalization, signal transduction, transcriptional regulation and DNA repair. In a screen for proteins associated with the nuclear envelope in the yeast Saccharomyces cerevisiae, we have identified the arginine methyltransferase Rmt2, previously shown to methylate the ribosomal protein L12. By indirect immunofluorescence and subcellular fractionations we demonstrate here that Rmt2 has nuclear and cytoplasmic localizations. Biochemical analysis of a fraction enriched in nuclei reveals that nuclear Rmt2 is resistant to extractions with salt and detergent, indicating an association with structural components. This was supported by affinity purification experiments with TAP-tagged Rmt2. Rmt2 was found to co-purify with the nucleoporins Nup49, Nup57 and Nup100, revealing a novel link between arginine methyltransferases and the nuclear pore complex. In addition, a genome-wide transcription study of the rmt2 Delta mutant shows significant downregulation of the transcription of MYO1, encoding the Type II myosin heavy chain required for cytokinesis and cell separation.

Place, publisher, year, edition, pages
2007. Vol. 313, no 9, 1778-1789 p.
National Category
Cancer and Oncology Cell Biology
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URN: urn:nbn:se:sh:diva-14224DOI: 10.1016/j.yexcr.2007.03.007ISI: 000246348400004PubMedID: 17448464ScopusID: 2-s2.0-35548947153OAI: oai:DiVA.org:sh-14224DiVA: diva2:467810
Available from: 2011-12-19 Created: 2011-12-19 Last updated: 2016-10-10Bibliographically approved

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Olsson, IdaBerrez, Jean-MarcMutvei, Ann
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