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Targeting cytokine expression in glial cells by cellular delivery of an NF-kappa B decoy
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2007 (English)In: Journal of Molecular Neuroscience, ISSN 0895-8696, E-ISSN 1559-1166, Vol. 31, no 3, 209-219 p.Article in journal (Refereed) Published
Abstract [en]

Inhibition of nuclear factor (NF)-kappa B has emerged as an important strategy for design of anti-inflammatory therapies. In neurodegenerative disorders like Alzheimer's disease, inflammatory reactions mediated by glial cells are believed to promote disease progression. Here, we report that uptake of a double-stranded oligonucleotide NF-kappa B decoy in rat primary glial cells is clearly facilitated by noncovalent binding to a cell-penetrating peptide, transportan 10, via a complementary peptide nucleic acid (PNA) sequence. Fluorescently labeled oligonucleotide decoy was detected in the cells within 1 h only when cells were incubated with the decoy in the presence of cell-penetrating peptide. Cellular delivery of the decoy also inhibited effects induced by a neurotoxic fragment of the Alzheimer beta-amyloid peptide in the presence of the inflammatory cytokine interleukin (IL)-1 beta. Pretreatment of the cells with the complex formed by the decoy and the cell-penetrating peptide-PNA resulted in 80% and 50% inhibition of the NF-kappa B binding activity and IL-6 mRNA expression, respectively.

Place, publisher, year, edition, pages
2007. Vol. 31, no 3, 209-219 p.
National Category
Biochemistry and Molecular Biology Neurology
Identifiers
URN: urn:nbn:se:sh:diva-14252DOI: 10.1385/JMN/31:03:209ISI: 000246588800003PubMedID: 17726227Scopus ID: 2-s2.0-34548336397OAI: oai:DiVA.org:sh-14252DiVA: diva2:467141
Available from: 2011-12-19 Created: 2011-12-19 Last updated: 2014-03-03Bibliographically approved

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Figueroa, RicardoHallberg, Einar
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CiteExportLink to record
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Citation style
  • apa
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  • modern-language-association-8th-edition
  • vancouver
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More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
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  • text
  • asciidoc
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