sh.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
Structural characterization of sialylated glycoforms of H-influenzae by electrospray mass spectrometry: fragmentation of protonated and sodiated O-deacylated lipopolysaccharides
Show others and affiliations
2007 (English)In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 21, no 6, 952-960 p.Article in journal (Refereed) Published
Abstract [en]

Sialylated lipopolysaccharide (LPS) glycoforms from Haemophilus influenzae were characterized by tandem mass spectrometry using a new generation hyphenated mass spectrometer which combines a triple quadrupole and a linear ion trap (Q-Trap). The fragmentation of both protonated and sodiated molecular ions from O-deacylated LPS (LPS-OH) obtained in MS2 experiments in the positive mode was studied. The MS2 spectra of protonated ions provided unambiguous evidence for the presence and sequence of sialylated lactosamine present in lacto-N-neotetraose oligosaccharide extensions but not for sialyl-lactose structures whilst fragmentation of sodiated adducts, [M+Na](+), afforded information diagnostic of mono- and disialylated lactose extensions. To study this we used a highly sialylated LPS from a H. influenzae strain capable of sialyl-lactose expression only. We then applied the method to the H. influenzae genome strain, Rd, in which glycoforms containing both sialyl-lactose and sialyl-lacto-N-neotetraose were detected from diagnostic B-ions at m/z 638.2 ([Neu5Ac(1) Hex(2)+ Na](+)) and 657.2 ([Neu5Ac(1) Hex(1) HexNAc(1)+H](+)). Unique fragmentation patterns provided the locations and sequences of these oligosaccharide extensions. This is the first time both sialylated lactose and sialylated lacto-N-neotetraose units have been detected and characterized by tandem mass spectrometry in the same molecule. This methodology is of general applicability for determination of common sialylated oligosaccharide extension in bacterial LPS.

Place, publisher, year, edition, pages
2007. Vol. 21, no 6, 952-960 p.
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:sh:diva-14255DOI: 10.1002/rcm.2916ISI: 000244923600016OAI: oai:DiVA.org:sh-14255DiVA: diva2:467137
Available from: 2011-12-19 Created: 2011-12-19 Last updated: 2011-12-19Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Schweda, Elke K. H.
By organisation
School of Life Sciences
In the same journal
Rapid Communications in Mass Spectrometry
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 46 hits
ReferencesLink to record
Permanent link

Direct link