sh.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
Duplicate copies of lic1 direct the addition of multiple phosphocholine residues in the lipopolysaccharide of Haemophilus influenzae
Södertörn University, School of Life Sciences.
Show others and affiliations
2008 (English)In: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 76, no 2, 588-600 p.Article in journal (Refereed) Published
Abstract [en]

The genes of the lic1 operon (lic1A to lic1D) are responsible for incorporation of phosphocholine (PCho) into the lipopolysaccharide (LPS) of Haemophilus influenzae. PCho plays a multifaceted role in the commensal and pathogenic lifestyles of a range of mucosal pathogens, including H. influenzae. Structural studies of the LPS of nontypeable H. influenzae (NTHI) have revealed that PCho can be linked to a hexose on any one of the oligosaccharide chain extensions from the conserved inner core triheptosyl backbone. In a collection of NTHI strains we found several strains in which there were two distinct but variant lic1D DNA sequences, genes predicted to encode the transferase responsible for directing the addition of PCho to LPS. The same isolates were also found to express concomitantly two PCho residues at distinct positions in their LPS. In one such NTHI isolate, isolate 1158, structural analysis of LPS from lic1 mutants confirmed that each of the two copies of lic1D directs the addition of PCho to a distinct location on the LPS. One position for PCho addition is a novel heptose, which is part of the oligosaccharide extension from the proximal heptose of the LPS inner core. Modification of the LPS by addition of two PCho residues resulted in increased binding of C-reactive protein and had consequential effects on the resistance of the organism to the killing effects of normal human serum compared to the effects of glycoforms containing one or no PCho. When bound, C-reactive protein leads to complement-mediated killing, indicating the potential biological significance of multiple PCho residues.

Place, publisher, year, edition, pages
2008. Vol. 76, no 2, 588-600 p.
National Category
Immunology
Identifiers
URN: urn:nbn:se:sh:diva-14169DOI: 10.1128/IAI.00748-07ISI: 000252978600015OAI: oai:DiVA.org:sh-14169DiVA: diva2:466980
Available from: 2011-12-18 Created: 2011-12-16 Last updated: 2011-12-18Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Schweda, Elke K. H.
By organisation
School of Life Sciences
In the same journal
Infection and Immunity
Immunology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 36 hits
ReferencesLink to record
Permanent link

Direct link