sh.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
Regulation of the Drosophila lin-41 homologue dappled by let-7 reveals conservation of a regulatory mechanism within the LIN-41 subclade
Södertörn University, School of Life Sciences. Karolinska Institute.
Södertörn University, School of Life Sciences.
2008 (English)In: Developmental Dynamics, ISSN 1058-8388, E-ISSN 1097-0177, Vol. 237, no 1, 196-208 p.Article in journal (Refereed) Published
Abstract [en]

Drosophila Dappled (DPLD) is a member of the RBCC/TRIM superfamily, a protein family involved in numerous diverse processes such as developmental timing and asymmetric cell divisions. DPLD belongs to the LIN-41 subclade, several members of which are micro RNA (miRNA) regulated. We re-examined the LIN-41 subclade members and their relation to other RBCC/TRIMs and dpld paralogs, and identified a new Drosophila muscle specific RBCC/TRIM: Another B-Box Affiliate, ABBA. In silico predictions of candidate miRNA regulators of dpld identified let-7 as the strongest candidate. Overexpression of dpld led to abnormal eye development, indicating that strict regulation of dpld mRNA levels is crucial for normal eye development. This phenotype was sensitive to let-7 dosage, suggesting let-7 regulation of dpld in the eye disc. A cell-based assay verified let-7 miRNA down-regulation of dpld expression by means of its 3'-untranslated region. Thus, dpld seems also to be miRNA regulated, suggesting that miRNAs represent an ancient mechanism of LIN-41 regulation.

Place, publisher, year, edition, pages
2008. Vol. 237, no 1, 196-208 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:sh:diva-14180DOI: 10.1002/dvdy.21396ISI: 000252386300019PubMedID: 18069688ScopusID: 2-s2.0-38149087481OAI: oai:DiVA.org:sh-14180DiVA: diva2:466971
Available from: 2011-12-18 Created: 2011-12-16 Last updated: 2014-02-27Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
O'Farrell, FergalKylsten, Per
By organisation
School of Life Sciences
In the same journal
Developmental Dynamics
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 32 hits
ReferencesLink to record
Permanent link

Direct link