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A Chromatin-remodeling Protein Is a Component of Fission Yeast Mediator
Södertörn University, School of Life Sciences, Molecular biology.
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2010 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 285, no 39, 29729-29737 p.Article in journal (Refereed) Published
Abstract [en]

The multiprotein Mediator complex is an important regulator of RNA polymerase II-dependent genes in eukaryotic cells. In contrast to the situation in many other eukaryotes, the conserved Med15 protein is not a stable component of Mediator isolated from fission yeast. We here demonstrate that Med15 exists in a protein complex together with Hrp1, a CHD1 ATP-dependent chromatin-remodeling protein. The Med15-Hrp1 subcomplex is not a component of the core Mediator complex but can interact with the L-Mediator conformation. Deletion of med15(+) and hrp1(+) causes very similar effects on global steady-state levels of mRNA, and genome-wide analyses demonstrate that Med15 associates with a distinct subset of Hrp1-bound gene promoters. Our findings therefore indicate that Mediator may directly influence histone density at regulated promoters.

Place, publisher, year, edition, pages
2010. Vol. 285, no 39, 29729-29737 p.
National Category
Biological Sciences
URN: urn:nbn:se:sh:diva-13969DOI: 10.1074/jbc.M110.153858ISI: 000281984300008ScopusID: 2-s2.0-77956924896OAI: diva2:466634
Available from: 2011-12-16 Created: 2011-12-15 Last updated: 2014-02-13Bibliographically approved

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