Conditions for dye-filling of sensory neurons in Caenorhabditis elegans
2010 (English)In: Journal of Neuroscience Methods, ISSN 0165-0270, E-ISSN 1872-678X, Vol. 188, no 1, 58-61 p.Article in journal (Refereed) Published
Dye-filling is a common method used to stain Caenorhabditis elegans ensory neurons in vivo. While the amphids and phasmids are easy to tain, a subset of sensory neurons, the IL2 neurons, are difficult to tain reproducibly. Here we examined the conditions under which the IL2 eurons take up the lipophilic fluorescent dye DiI. We find that IL2 ye-filling depends on salt concentration, but not osmolarity. Low salt rior and during incubation is important for efficient dye uptake. dditional parameters that affect dye-filling are the speed of shaking uring incubation and the addition of detergents. Our modified ye-filling procedure provides a reliable method to distinguish mutant lleles that stain amphids and phasmids, IL2 neurons, or both. An dditional benefit is that it can also stain the excretory duct. The ethod allows genetic screens to be performed to identify mutants that electively affect only one of the sensory structures or the excretory uct.
Place, publisher, year, edition, pages
2010. Vol. 188, no 1, 58-61 p.
IdentifiersURN: urn:nbn:se:sh:diva-13709DOI: 10.1016/j.jneumeth.2010.02.003ISI: 000276866400009PubMedID: 20149821ScopusID: 2-s2.0-77950460160OAI: oai:DiVA.org:sh-13709DiVA: diva2:462203