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Characterization of the bifunctional mitochondrial processing peptidase (MPP)/bc1 complex in Spinacia oleracea
1996 (English)In: Journal of Bioenergetics and Biomembranes, ISSN 0145-479X, E-ISSN 1573-6881, Vol. 28, no 3, 285-292 p.Article in journal (Refereed) Published
Abstract [en]

The mitochondrial general processing peptidase (MPP) in plant mitochondria constitutes an integral part of the cytochromebc 1 complex of the respiratory chain. Here we present a characterization of this bifunctional complex from spinach leaf mitochondria. The purified MPP/bc 1 complex has a molecular mass of 550 kDa, which corresponds to a dimer. Increased ionic strength results in partial dissociation of the dimer as well as loss of the processing activity. Micellar concentrations of nonionic and zwitterionic detergents stimulate the activity by decreasing the temperature optimum of the processing reaction, whereas anionic detergents totally suppress the activity. MPP is a metalloendopeptidase. Interestingly, hemin, a potent regulator of mitochondrial and cytosolic biogenesis and inhibitor of proteosomal degradation, inhibits the processing activity. Measurements of the processing activity at different redox states of the bc 1 complex show that despite bifunctionality of the MPP/bc 1 complex, there is no correlation between electron transfer and protein processing.

Place, publisher, year, edition, pages
1996. Vol. 28, no 3, 285-292 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:sh:diva-8722DOI: 10.1007/BF02110702OAI: oai:DiVA.org:sh-8722DiVA: diva2:427713
Available from: 2011-06-28 Created: 2011-05-27 Last updated: 2016-10-07Bibliographically approved

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Sjöling, Sara
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