Mitochondrial protein import in plants: Signals, Sorting, Targeting, Processing and Regulation
1998 (English)In: Plant Molecular Biology, ISSN 0167-4412, E-ISSN 1573-5028, Vol. 38, no 1-2, 311-338 p.Article in journal (Refereed) Published
Mitochondrial biogenesis requires a coordinated expression of both the nuclear and the organellar genomes and specific intracellular protein trafficking, processing and assembly machinery. Mostmitochondrial proteins are synthesised as precursor proteins containing an N-terminal extension which functions as a targeting signal, which is proteolytically cleaved off after import into mitochondria. We review our present knowledge on components and mechanisms involved in the mitochondrial proteinimport process in plants. This encompasses properties of targeting peptides, sorting of precursor proteinsbetween mitochondria and chloroplasts, signal recognition, mechanism of translocation across the mitochondrial membranes and the role of cytosolic and organellar molecular chaperones in this process. The mitochondrial protein processing in plants is catalysed by the mitochondrial processing peptidase (MPP), which in contrast to other sources, is integrated into the bc1 complex of the respiratory chain. This is the most studied component of the plant import machinery characterised to date. What are the biochemical consequences of the integration of the MPP into an oligomeric protein complex and how are several hundred presequences of precursor proteins with no sequence similarities and no consensus for cleavage, specifically cleaved off by MPP? Finally we will address the emerging area of the control of protein import into mitochondria.
Place, publisher, year, edition, pages
1998. Vol. 38, no 1-2, 311-338 p.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:sh:diva-8729DOI: 10.1023/A:1006020208140OAI: oai:DiVA.org:sh-8729DiVA: diva2:427663