sh.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
Characterization of Rhizobacteria isolated from Wild Coffea arabica L.
Södertörn University, School of Life Sciences, Molecular biology.
Show others and affiliations
2009 (English)In: Engineering in Life Sciences, ISSN 1618-0240, E-ISSN 1618-2863, Vol. 9, no 2, 100-108 p.Article in journal (Refereed) Published
Abstract [en]

Rhizobacteria from wild Arabica coffee Populations (Coffea arabica L.) in southwestern Ethiopia were isolated and characterized. The main purpose was to identify coffee-associated rhizobacteria and evaluate their potential in synthesizing the phytohormone indole acetic acid (IAA) and in degrading the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC). A total of 878 bacterial isolates were screened, of which 395 (45%) isolates were preliminarily characterized using metabolic identification kits (API). Both Gram-negative and Gram-positive bacteria were isolated, with the former group predominating (63% of cases). Based on pre-screening results of the biochemical tests, 51 of the isolates were subjected to PCR-RFLP (Restriction Fragment Length Polymorphism) analysis that yielded ten groups, of which 24 isolates were identified by 16S rRNA gene sequencing. The major genera identified were Pseudomonas (six species) and Bacillus (four species). Single species of Erwinia, Ochrobactrum and Serratia were also identified. The Erwinia sp., Serratia marcescens and many Pseudomonas spp. produced IAA, and some isolates (all Pseudomonas spp.) were also able to degrade ACC. Several of the microbes found in association with wild Arabica coffee bushes have potential agronomic importance, like e.g. Bacillus thuringiensis, which deserve further testing. According to these in vitro Studies, Isolates of Erwinia, Serratia and Pseudomonas are of particular interest in inoculant development due to their plant growth promoting traits.

Place, publisher, year, edition, pages
2009. Vol. 9, no 2, 100-108 p.
Keyword [en]
ACC, Coffee populations, Native rhizobacteria, Phytohormones
National Category
Microbiology
Identifiers
URN: urn:nbn:se:sh:diva-8345DOI: 10.1002/elsc.200700031ISI: 000265855200002ScopusID: 2-s2.0-66449119071OAI: oai:DiVA.org:sh-8345DiVA: diva2:414708
Available from: 2011-05-04 Created: 2011-05-04 Last updated: 2015-06-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Hjort, Karin
By organisation
Molecular biology
In the same journal
Engineering in Life Sciences
Microbiology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 51 hits
ReferencesLink to record
Permanent link

Direct link