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Microbial community structure in polluted Baltic Sea sediments
Södertörn University, School of Life Sciences. SLU.
Södertörn University, School of Life Sciences.ORCID iD: 0000-0002-5802-5126
2006 (English)In: Environmental Microbiology, ISSN 1462-2912, E-ISSN 1462-2920, Vol. 8, no 2, 223-232 p.Article in journal (Refereed) Published
Abstract [en]

Nearly half the seabed of the Baltic Proper is incapable of supporting life of higher organisms as a consequence of oxygen depletion resulting from eutrophication. However, these areas are actually teeming with microbial life. Here we used terminal-restriction fragment length polymorphism (T-RFLP) to investigate the dominant archaeal and bacterial groups, with respect to community structure, in surface layers of bottom sediments of the Baltic Sea along a coastal pollution gradient. Both archaeal and bacterial communities formed distinct clusters along the pollution gradient and the community compositions were different at the polluted sites compared with the relatively clean reference sites. The structures of the bacterial communities were most strongly correlated to water depth, followed by organic carbon, oxygen, salinity and silicate levels. In contrast, the structures of the archaeal communities were most strongly correlated to oxygen, salinity, organic carbon, silicate and nitrate levels. Some members of the microbial communities were identified using a combination of traditional and molecular approaches. Isolates obtained on different culture media were identified by partial sequencing of their 16S rRNA genes and some novel species were found. In addition, we developed a computer program, APLAUS, to elucidate the putative identities of the most dominant community members by T-RFLP.

Place, publisher, year, edition, pages
United Kingdom: Wiley-Blackwell Publishing Ltd. , 2006. Vol. 8, no 2, 223-232 p.
Keyword [en]
RIBOSOMAL-RNA; MARINE BACTERIOPLANKTON; DIVERSITY; EUTROPHICATION; ANTARCTICA; SALINITY; BACTERIAL; DYNAMICS; ARCHAEA; WATERS
National Category
Natural Sciences
Research subject
Environmental Studies
Identifiers
URN: urn:nbn:se:sh:diva-6194DOI: 10.1111/j.1462-2920.2005.00887.xISI: 000234647600005PubMedID: 16423011Scopus ID: 2-s2.0-33644870711OAI: oai:DiVA.org:sh-6194DiVA: diva2:397122
Available from: 2011-02-11 Created: 2011-02-11 Last updated: 2016-12-01Bibliographically approved
In thesis
1. Microbial diversity in Baltic Sea sediments
Open this publication in new window or tab >>Microbial diversity in Baltic Sea sediments
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis focuses on microbial community structures and their functions in Baltic Sea sediments. First we investigated the distribution of archaea and bacteria in Baltic Sea sediments along a eutrophication gradient. Community profile analysis of 16S rRNA genes using terminal restriction length polymorphism (T-RFLP) indicated that archaeal and bacterial communities were spatially heterogeneous. By employing statistical ordination methods we observed that archaea and bacteria were structured and impacted differently by environmental parameters that were significantly linked to eutrophication. In a separate study, we analyzed bacterial communities at a different site in the Baltic Sea that was heavily contaminated with polyaromatic hydrocarbons (PAHs) and several other pollutants. Sediment samples were collected before and after remediation by dredging in two consecutive years. A polyphasic experimental approach was used to assess growing bacteria and degradation genes in the sediments. The bacterial communities were significantly different before and after dredging of the sediment. Several isolates collected from contaminated sediments showed an intrinsic capacity for degradation of phenanthrene (a PAH model compound). Quantititative real-time PCR was used to monitor the abundance of degradation genes in sediment microcosms spiked with phenanthrene. Although both xylE and phnAc genes increased in abundance in the microcosms, the isolates only carried phnAc genes. Isolates with closest 16S rRNA gene sequence matches to Exigobacterium oxidotolerans, a Pseudomonas sp. and a Gammaproteobacterium were identified by all approaches used as growing bacteria that are capable of phenanthrene degradation. These isolates were assigned species and strain designations as follows: Exiguobacterium oxidotolerans AE3, Pseudomonas fluorescens AE1 and Pseudomonas migulae AE2. We also identified and studied the distribution of actively growing bacteria along red-ox profiles in Baltic Sea sediments. Community structures were found to be significantly different at different red-ox depths. Also, according to multivariate statistical ordination analysis organic carbon, nitrogen, and red-ox potential were crucial parameters for structuring the bacterial communities on a vertical scale. Novel lineages of bacteria were obtained by sequencing 16S rRNA genes from different red-ox depths and sampling stations indicating that bacterial diversity in Baltic Sea sediments is largely unexplored.

Place, publisher, year, edition, pages
Uppsala: Sveriges Lantbruksuniversitet, 2007. 36 p.
Series
Acta Universitatis agriculturae Sueciae, ISSN 1652-6880 ; 2007:6
Keyword
Havsbottnen, Mikroorganismer
National Category
Biological Sciences
Research subject
Baltic and East European studies
Identifiers
urn:nbn:se:sh:diva-31261 (URN)91-576-7325-X (ISBN)
Supervisors
Available from: 2016-12-01 Created: 2016-12-01 Last updated: 2016-12-01Bibliographically approved

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Citation style
  • apa
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